1n6q: Difference between revisions
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New page: left|200px<br /> <applet load="1n6q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n6q, resolution 3.Å" /> '''HIV-1 Reverse Transcr... |
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[[Image:1n6q.gif|left|200px]]<br /> | [[Image:1n6q.gif|left|200px]]<br /><applet load="1n6q" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1n6q" size=" | |||
caption="1n6q, resolution 3.Å" /> | caption="1n6q, resolution 3.Å" /> | ||
'''HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)'''<br /> | '''HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)'''<br /> | ||
==Overview== | ==Overview== | ||
AZT (3'-azido-3'-deoxythymidine) resistance involves the enhanced excision | AZT (3'-azido-3'-deoxythymidine) resistance involves the enhanced excision of AZTMP from the end of the primer strand by HIV-1 reverse transcriptase. This reaction can occur when an AZTMP-terminated primer is bound at the nucleotide-binding site (pre-translocation complex N) but not at the 'priming' site (post-translocation complex P). We determined the crystal structures of N and P complexes at 3.0 and 3.1 A resolution. These structures provide insight into the structural basis of AZTMP excision and the mechanism of translocation. Docking of a dNTP in the P complex structure suggests steric crowding in forming a stable ternary complex that should increase the relative amount of the N complex, which is the substrate for excision. Structural differences between complexes N and P suggest that the conserved YMDD loop is involved in translocation, acting as a springboard that helps to propel the primer terminus from the N to the P site after dNMP incorporation. | ||
==About this Structure== | ==About this Structure== | ||
1N6Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http:// | 1N6Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6Q OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: RNA-directed DNA polymerase]] | [[Category: RNA-directed DNA polymerase]] | ||
[[Category: Arnold, E.]] | [[Category: Arnold, E.]] | ||
[[Category: Birktoft, J | [[Category: Birktoft, J J.]] | ||
[[Category: Boyer, P | [[Category: Boyer, P L.]] | ||
[[Category: Das, K.]] | [[Category: Das, K.]] | ||
[[Category: Hughes, S | [[Category: Hughes, S H.]] | ||
[[Category: Ilankumaran, I.]] | [[Category: Ilankumaran, I.]] | ||
[[Category: Jerina, D | [[Category: Jerina, D M.]] | ||
[[Category: Jr., A | [[Category: Jr., A D.Clark.]] | ||
[[Category: Ramesha, A | [[Category: Ramesha, A R.]] | ||
[[Category: Sarafianos, S | [[Category: Sarafianos, S G.]] | ||
[[Category: Sayer, J | [[Category: Sayer, J M.]] | ||
[[Category: Tuske, S.]] | [[Category: Tuske, S.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: translocation]] | [[Category: translocation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:50 2008'' | ||
