1n7s: Difference between revisions

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[[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1n7s, resolution 1.45Å" />

'''High Resolution Structure of a Truncated Neuronal SNARE Complex'''<br />

==Overview==

The structure of a truncated SNARE complex has been solved to 1.4-A, resolution revealing a stabilizing salt bridge, sites of hydration, and, conformational variability of the ionic central layer that were not, observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid, binding and denatures at a lower temperature than longer complexes as, assessed by SDS and circular dichroism thermal melts. The truncated SNARE, complex is monomeric, and it retains binding to synaptotagmin I.

The structure of a truncated SNARE complex has been solved to 1.4-A resolution revealing a stabilizing salt bridge, sites of hydration, and conformational variability of the ionic central layer that were not observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid binding and denatures at a lower temperature than longer complexes as assessed by SDS and circular dichroism thermal melts. The truncated SNARE complex is monomeric, and it retains binding to synaptotagmin I.

==About this Structure==

1N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA].

1N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Rattus norvegicus]]

[[Category: Brunger, A.T.]]

[[Category: Brunger, A T.]]

[[Category: Ernst, J.A.]]

[[Category: Ernst, J A.]]

[[Category: CA]]

[[Category: MPD]]

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[[Category: neuronal snare protein complex]]

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@@ Line 1: / Line 1: @@
-[[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n7s, resolution 1.45&Aring;" />
 '''High Resolution Structure of a Truncated Neuronal SNARE Complex'''<br />
 ==Overview==
-The structure of a truncated SNARE complex has been solved to 1.4-A, resolution revealing a stabilizing salt bridge, sites of hydration, and, conformational variability of the ionic central layer that were not, observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid, binding and denatures at a lower temperature than longer complexes as, assessed by SDS and circular dichroism thermal melts. The truncated SNARE, complex is monomeric, and it retains binding to synaptotagmin I.
+The structure of a truncated SNARE complex has been solved to 1.4-A resolution revealing a stabilizing salt bridge, sites of hydration, and conformational variability of the ionic central layer that were not observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid binding and denatures at a lower temperature than longer complexes as assessed by SDS and circular dichroism thermal melts. The truncated SNARE complex is monomeric, and it retains binding to synaptotagmin I.
 ==About this Structure==
-N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA].
+N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Rattus norvegicus]]
-[[Category: Brunger, A.T.]]
+[[Category: Brunger, A T.]]
-[[Category: Ernst, J.A.]]
+[[Category: Ernst, J A.]]
 [[Category: CA]]
 [[Category: MPD]]
@@ Line 20: / Line 20: @@
 [[Category: neuronal snare protein complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:01:51 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:08 2008''