1nhv: Difference between revisions
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New page: left|200px<br /><applet load="1nhv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nhv, resolution 2.90Å" /> '''Hepatitis C virus RN... |
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[[Image:1nhv.gif|left|200px]]<br /><applet load="1nhv" size=" | [[Image:1nhv.gif|left|200px]]<br /><applet load="1nhv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nhv, resolution 2.90Å" /> | caption="1nhv, resolution 2.90Å" /> | ||
'''Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor'''<br /> | '''Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor'''<br /> | ||
==Overview== | ==Overview== | ||
X-ray crystal structures of two non-nucleoside analogue inhibitors bound | X-ray crystal structures of two non-nucleoside analogue inhibitors bound to hepatitis C virus NS5B RNA-dependent RNA polymerase have been determined to 2.0 and 2.9 A resolution. These noncompetitive inhibitors bind to the same site on the protein, approximately 35 A from the active site. The common features of binding include a large hydrophobic region and two hydrogen bonds between both oxygen atoms of a carboxylate group on the inhibitor and two main chain amide nitrogen atoms of Ser(476) and Tyr(477) on NS5B. The inhibitor-binding site lies at the base of the thumb domain, near its interface with the C-terminal extension of NS5B. The location of this inhibitor-binding site suggests that the binding of these inhibitors interferes with a conformational change essential for the activity of the polymerase. | ||
==About this Structure== | ==About this Structure== | ||
1NHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with 154 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | 1NHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with <scene name='pdbligand=154:'>154</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Alaoui-Ismaili, M | [[Category: Alaoui-Ismaili, M H.]] | ||
[[Category: Bedard, J.]] | [[Category: Bedard, J.]] | ||
[[Category: Bethell, R | [[Category: Bethell, R C.]] | ||
[[Category: Chan, L.]] | [[Category: Chan, L.]] | ||
[[Category: Cherney, M | [[Category: Cherney, M M.]] | ||
[[Category: James, M | [[Category: James, M N.G.]] | ||
[[Category: Morin, N.]] | [[Category: Morin, N.]] | ||
[[Category: Ng, K | [[Category: Ng, K K.S.]] | ||
[[Category: Nguyen-Ba, N.]] | [[Category: Nguyen-Ba, N.]] | ||
[[Category: Wang, M.]] | [[Category: Wang, M.]] | ||
[[Category: Yannopoulos, C | [[Category: Yannopoulos, C G.]] | ||
[[Category: 154]] | [[Category: 154]] | ||
[[Category: hepatitis c; rna polymerase; enzyme inhibition]] | [[Category: hepatitis c; rna polymerase; enzyme inhibition]] | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:15 2008'' | ||
