1not: Difference between revisions

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[[Image:1not.gif|left|200px]]<br /><applet load="1not" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1not.gif|left|200px]]<br /><applet load="1not" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1not, resolution 1.2Å" />

'''THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN'''<br />

==Overview==

Predatory marine snails of the genus Conus paralyze their fish prey by, injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide, isolated from venom of Conus geographus. It functions by blocking the, postsynaptic nicotinic acetylcholine receptor. After crystallization in, deionized water, the three-dimensional structure of the GI neurotoxin was, determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to, those derived by NMR, CD, and predictive methods. The principal framework, of the molecule is provided by two disulfide bonds, one linking Cys 2 and, Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are, drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and, between Cys 2 and Ser 12. Since the C-terminus is amidated, only one, negative charge is present (carboxylate of Glu 1), and this is not, implicated in receptor binding. Two positively charged regions (the, alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15, A apart at the corners of the triangular face of the molecule. phi, psi, angles characteristic of a 3(10) helix were observed for residues 5-7. For, residues 8-11, these angles were consistent with either a type I beta-turn, or a distorted 3(10) helix.

Predatory marine snails of the genus Conus paralyze their fish prey by injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide isolated from venom of Conus geographus. It functions by blocking the postsynaptic nicotinic acetylcholine receptor. After crystallization in deionized water, the three-dimensional structure of the GI neurotoxin was determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to those derived by NMR, CD, and predictive methods. The principal framework of the molecule is provided by two disulfide bonds, one linking Cys 2 and Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and between Cys 2 and Ser 12. Since the C-terminus is amidated, only one negative charge is present (carboxylate of Glu 1), and this is not implicated in receptor binding. Two positively charged regions (the alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15 A apart at the corners of the triangular face of the molecule. phi, psi angles characteristic of a 3(10) helix were observed for residues 5-7. For residues 8-11, these angles were consistent with either a type I beta-turn or a distorted 3(10) helix.

==About this Structure==

1NOT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1NOT OCA].

1NOT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOT OCA].

==Reference==

Line 13:

[[Category: Conus geographus]]

[[Category: Single protein]]

[[Category: Edmundson, A.B.]]

[[Category: Edmundson, A B.]]

[[Category: Gray, W.R.]]

[[Category: Gray, W R.]]

[[Category: Guddat, L.W.]]

[[Category: Guddat, L W.]]

[[Category: Martin, J.L.]]

[[Category: Martin, J L.]]

[[Category: Shan, L.]]

[[Category: NH2]]

Line 24:

[[Category: venom]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 22~~:26:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:24 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1not.gif|left|200px]]<br /><applet load="1not" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1not.gif|left|200px]]<br /><applet load="1not" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1not, resolution 1.2&Aring;" />
 '''THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN'''<br />
 ==Overview==
-Predatory marine snails of the genus Conus paralyze their fish prey by, injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide, isolated from venom of Conus geographus. It functions by blocking the, postsynaptic nicotinic acetylcholine receptor. After crystallization in, deionized water, the three-dimensional structure of the GI neurotoxin was, determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to, those derived by NMR, CD, and predictive methods. The principal framework, of the molecule is provided by two disulfide bonds, one linking Cys 2 and, Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are, drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and, between Cys 2 and Ser 12. Since the C-terminus is amidated, only one, negative charge is present (carboxylate of Glu 1), and this is not, implicated in receptor binding. Two positively charged regions (the, alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15, A apart at the corners of the triangular face of the molecule. phi, psi, angles characteristic of a 3(10) helix were observed for residues 5-7. For, residues 8-11, these angles were consistent with either a type I beta-turn, or a distorted 3(10) helix.
+Predatory marine snails of the genus Conus paralyze their fish prey by injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide isolated from venom of Conus geographus. It functions by blocking the postsynaptic nicotinic acetylcholine receptor. After crystallization in deionized water, the three-dimensional structure of the GI neurotoxin was determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to those derived by NMR, CD, and predictive methods. The principal framework of the molecule is provided by two disulfide bonds, one linking Cys 2 and Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and between Cys 2 and Ser 12. Since the C-terminus is amidated, only one negative charge is present (carboxylate of Glu 1), and this is not implicated in receptor binding. Two positively charged regions (the alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15 A apart at the corners of the triangular face of the molecule. phi, psi angles characteristic of a 3(10) helix were observed for residues 5-7. For residues 8-11, these angles were consistent with either a type I beta-turn or a distorted 3(10) helix.
 ==About this Structure==
-NOT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOT OCA].
+NOT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOT OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Conus geographus]]
 [[Category: Single protein]]
-[[Category: Edmundson, A.B.]]
+[[Category: Edmundson, A B.]]
-[[Category: Gray, W.R.]]
+[[Category: Gray, W R.]]
-[[Category: Guddat, L.W.]]
+[[Category: Guddat, L W.]]
-[[Category: Martin, J.L.]]
+[[Category: Martin, J L.]]
 [[Category: Shan, L.]]
 [[Category: NH2]]
@@ Line 24: / Line 24: @@
 [[Category: venom]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:26:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:24 2008''