1o7z: Difference between revisions

Line 4:

==Overview==

We have determined the structure of wild-type IP-10 from three crystal, forms. The crystals provide eight separate models of the IP-10 chain, all, differing substantially from a monomeric IP-10 variant examined previously, by NMR spectroscopy. In each crystal form, IP-10 chains form conventional, beta sheet dimers, which, in turn, form a distinct tetrameric assembly., The M form tetramer is reminiscent of platelet factor 4, whereas the T and, H forms feature a novel twelve-stranded beta sheet. Analytical, ultracentrifugation indicates that, in free solution, IP-10 exists in a, monomer-dimer equilibrium with a dissociation constant of 9 microM. We, propose that the tetrameric structures may represent species promoted by, the binding of glycosaminoglycans. The binding sites for several, IP-10-neutralizing mAbs have also been mapped.

We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.

==About this Structure==

Line 12:

Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737818 12737818]

[[Category: Single protein]]

[[Category: Acharya, K.R.]]

[[Category: Acharya, K R.]]

[[Category: Holloway, D.E.]]

[[Category: Holloway, D E.]]

[[Category: Papageorgiou, A.C.]]

[[Category: Papageorgiou, A C.]]

[[Category: Swaminathan, G.J.]]

[[Category: Swaminathan, G J.]]

[[Category: chemokine]]

[[Category: chemotaxis]]

Line 21:

[[Category: interferon induction]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb ~~15 16~~:33:19 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:14:31 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-We have determined the structure of wild-type IP-10 from three crystal, forms. The crystals provide eight separate models of the IP-10 chain, all, differing substantially from a monomeric IP-10 variant examined previously, by NMR spectroscopy. In each crystal form, IP-10 chains form conventional, beta sheet dimers, which, in turn, form a distinct tetrameric assembly., The M form tetramer is reminiscent of platelet factor 4, whereas the T and, H forms feature a novel twelve-stranded beta sheet. Analytical, ultracentrifugation indicates that, in free solution, IP-10 exists in a, monomer-dimer equilibrium with a dissociation constant of 9 microM. We, propose that the tetrameric structures may represent species promoted by, the binding of glycosaminoglycans. The binding sites for several, IP-10-neutralizing mAbs have also been mapped.
+We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737818 12737818]
 [[Category: Single protein]]
-[[Category: Acharya, K.R.]]
+[[Category: Acharya, K R.]]
-[[Category: Holloway, D.E.]]
+[[Category: Holloway, D E.]]
-[[Category: Papageorgiou, A.C.]]
+[[Category: Papageorgiou, A C.]]
-[[Category: Swaminathan, G.J.]]
+[[Category: Swaminathan, G J.]]
 [[Category: chemokine]]
 [[Category: chemotaxis]]
@@ Line 21: / Line 21: @@
 [[Category: interferon induction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:33:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:14:31 2008''