1obw: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1obw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1obw, resolution 1.9Å" /> '''STRUCTURE OF INORGANI... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1obw.jpg|left|200px]]<br /><applet load="1obw" size=" | [[Image:1obw.jpg|left|200px]]<br /><applet load="1obw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1obw, resolution 1.9Å" /> | caption="1obw, resolution 1.9Å" /> | ||
'''STRUCTURE OF INORGANIC PYROPHOSPHATASE'''<br /> | '''STRUCTURE OF INORGANIC PYROPHOSPHATASE'''<br /> | ||
==Overview== | ==Overview== | ||
Crystalline holo inorganic pyrophosphatase from Escherichia coli was grown | Crystalline holo inorganic pyrophosphatase from Escherichia coli was grown in the presence of 250 mM MgCl2. The crystal structure has been solved by Patterson search techniques and refined to an R-factor of 17.6% at 1.9 A resolution. The upper estimate of the root-mean-square error in atomic positions is 0.26 A. These crystals belong to space group P3(2)21 with unit cell dimensions a = b = 110.27 A and c = 78.17 A. The asymmetric unit contains a trimer of subunits, i.e., half of the hexameric molecule. In the central cavity of the enzyme molecule, three Mg2+ ions, each shared by two subunits of the hexamer, are found. In the active sites of two crystallographically independent subunits, two Mg2+ ions are bound. The second active site Mg2+ ion is missing in the third subunit. A mechanism of catalysis is proposed whereby a water molecule activated by a Mg2+ ion and Tyr 55 play essential roles. | ||
==About this Structure== | ==About this Structure== | ||
1OBW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http:// | 1OBW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBW OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Inorganic diphosphatase]] | [[Category: Inorganic diphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Avaeva, S | [[Category: Avaeva, S M.]] | ||
[[Category: Harutyunyan, E | [[Category: Harutyunyan, E H.]] | ||
[[Category: Huber, R.]] | [[Category: Huber, R.]] | ||
[[Category: Mather, T.]] | [[Category: Mather, T.]] | ||
[[Category: Oganessyan, N | [[Category: Oganessyan, N N.]] | ||
[[Category: Oganessyan, V | [[Category: Oganessyan, V Yu.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: 3d-structure]] | [[Category: 3d-structure]] | ||
| Line 26: | Line 26: | ||
[[Category: metal binding]] | [[Category: metal binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:15:54 2008'' | ||
