1ogy: Difference between revisions

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==Overview==

The structure of the respiratory nitrate reductase (NapAB) from, Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible, for the first step in the denitrification process, has been determined at, a resolution of 3.2 A. The di-heme electron transfer small subunit NapB, binds to the large subunit with heme II in close proximity to the [4Fe-4S], cluster of NapA. A total of 57 residues at the N- and C-terminal, extremities of NapB adopt an extended conformation, embracing the NapA, subunit and largely contributing to the total area of 5,900 A(2) buried in, the complex. Complex formation was studied further by measuring the, variation of the redox potentials of all the cofactors upon binding. The, marked effects observed are interpreted in light of the three-dimensional, structure and depict a plasticity that contributes to an efficient, electron transfer in the complex from the heme I of NapB to the molybdenum, catalytic site of NapA.

The structure of the respiratory nitrate reductase (NapAB) from Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible for the first step in the denitrification process, has been determined at a resolution of 3.2 A. The di-heme electron transfer small subunit NapB binds to the large subunit with heme II in close proximity to the [4Fe-4S] cluster of NapA. A total of 57 residues at the N- and C-terminal extremities of NapB adopt an extended conformation, embracing the NapA subunit and largely contributing to the total area of 5,900 A(2) buried in the complex. Complex formation was studied further by measuring the variation of the redox potentials of all the cofactors upon binding. The marked effects observed are interpreted in light of the three-dimensional structure and depict a plasticity that contributes to an efficient electron transfer in the complex from the heme I of NapB to the molybdenum catalytic site of NapA.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Rhodobacter sphaeroides]]

[[Category: Adriano, J.M.]]

[[Category: Adriano, J M.]]

[[Category: Alric, J.]]

[[Category: Arnoux, P.]]

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[[Category: oxidoreductase]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The structure of the respiratory nitrate reductase (NapAB) from, Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible, for the first step in the denitrification process, has been determined at, a resolution of 3.2 A. The di-heme electron transfer small subunit NapB, binds to the large subunit with heme II in close proximity to the [4Fe-4S], cluster of NapA. A total of 57 residues at the N- and C-terminal, extremities of NapB adopt an extended conformation, embracing the NapA, subunit and largely contributing to the total area of 5,900 A(2) buried in, the complex. Complex formation was studied further by measuring the, variation of the redox potentials of all the cofactors upon binding. The, marked effects observed are interpreted in light of the three-dimensional, structure and depict a plasticity that contributes to an efficient, electron transfer in the complex from the heme I of NapB to the molybdenum, catalytic site of NapA.
+The structure of the respiratory nitrate reductase (NapAB) from Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible for the first step in the denitrification process, has been determined at a resolution of 3.2 A. The di-heme electron transfer small subunit NapB binds to the large subunit with heme II in close proximity to the [4Fe-4S] cluster of NapA. A total of 57 residues at the N- and C-terminal extremities of NapB adopt an extended conformation, embracing the NapA subunit and largely contributing to the total area of 5,900 A(2) buried in the complex. Complex formation was studied further by measuring the variation of the redox potentials of all the cofactors upon binding. The marked effects observed are interpreted in light of the three-dimensional structure and depict a plasticity that contributes to an efficient electron transfer in the complex from the heme I of NapB to the molybdenum catalytic site of NapA.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Rhodobacter sphaeroides]]
-[[Category: Adriano, J.M.]]
+[[Category: Adriano, J M.]]
 [[Category: Alric, J.]]
 [[Category: Arnoux, P.]]
@@ Line 28: / Line 28: @@
 [[Category: oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:58:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:38 2008''