1omy: Difference between revisions

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[[Image:1omy.jpg|left|200px]]<br /><applet load="1omy" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1omy.jpg|left|200px]]<br /><applet load="1omy" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1omy, resolution 2.00Å" />

'''Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch'''<br />

==Overview==

Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins, for which no crystal structures are yet available. A novel alpha-insect, toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK), has been expressed as an inclusion body in Escherichia coli and purified, by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1), was crystallized using the vapour-diffusion technique in hanging drops at, 296 K. The crystals, which were grown in sodium phosphate, belonged to the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected, to 2.1 A resolution using synchrotron radiation. There appears to be one, rBmKalphaIT1 molecule in the asymmetric unit.

Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.

==About this Structure==

1OMY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii] with CL and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OMY OCA].

1OMY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OMY OCA].

==Reference==

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[[Category: sodium channel]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:01:~~14 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:34 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1omy.jpg|left|200px]]<br /><applet load="1omy" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1omy.jpg|left|200px]]<br /><applet load="1omy" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1omy, resolution 2.00&Aring;" />
 '''Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch'''<br />
 ==Overview==
-Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins, for which no crystal structures are yet available. A novel alpha-insect, toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK), has been expressed as an inclusion body in Escherichia coli and purified, by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1), was crystallized using the vapour-diffusion technique in hanging drops at, 296 K. The crystals, which were grown in sodium phosphate, belonged to the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected, to 2.1 A resolution using synchrotron radiation. There appears to be one, rBmKalphaIT1 molecule in the asymmetric unit.
+Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.
 ==About this Structure==
-OMY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii] with CL and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OMY OCA].
+OMY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OMY OCA].
 ==Reference==
@@ Line 24: / Line 24: @@
 [[Category: sodium channel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:01:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:34 2008''