1oy2: Difference between revisions
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==Overview== | ==Overview== | ||
The notion that certain proteins lack intrinsic globular structure under | The notion that certain proteins lack intrinsic globular structure under physiological conditions and that the attainment of fully folded structure only occurs upon the binding of target molecules has been recently gaining popularity. We report here the solution structure of the PTB domain of the signaling protein Shc in the free form. Comparison of this structure with that of the complex form, obtained previously with a phosphopeptide ligand, reveals that the Shc PTB domain is structurally disordered in the free form, particularly around the regions constituting the peptide binding pocket. The binding of the ligand appears to reorganize this pocket through local folding events triggering a conformational switch between the free and the complex forms. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Farooq, A.]] | [[Category: Farooq, A.]] | ||
[[Category: Ravichandran, K | [[Category: Ravichandran, K S.]] | ||
[[Category: Yan, K | [[Category: Yan, K S.]] | ||
[[Category: Zeng, L.]] | [[Category: Zeng, L.]] | ||
[[Category: Zhou, M | [[Category: Zhou, M M.]] | ||
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: free protein]] | [[Category: free protein]] | ||
[[Category: structure disorder]] | [[Category: structure disorder]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:56 2008'' | ||
Revision as of 12:22, 21 February 2008
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Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc
Overview
The notion that certain proteins lack intrinsic globular structure under physiological conditions and that the attainment of fully folded structure only occurs upon the binding of target molecules has been recently gaining popularity. We report here the solution structure of the PTB domain of the signaling protein Shc in the free form. Comparison of this structure with that of the complex form, obtained previously with a phosphopeptide ligand, reveals that the Shc PTB domain is structurally disordered in the free form, particularly around the regions constituting the peptide binding pocket. The binding of the ligand appears to reorganize this pocket through local folding events triggering a conformational switch between the free and the complex forms.
About this Structure
1OY2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Coupling of folding and binding in the PTB domain of the signaling protein Shc., Farooq A, Zeng L, Yan KS, Ravichandran KS, Zhou MM, Structure. 2003 Aug;11(8):905-13. PMID:12906822
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