1oy8: Difference between revisions

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==Overview==

Multidrug efflux pumps cause serious problems in cancer chemotherapy and, treatment of bacterial infections. Yet high-resolution structures of, ligand transporter complexes have previously been unavailable. We obtained, x-ray crystallographic structures of the trimeric AcrB pump from, Escherichia coli with four structurally diverse ligands. The structures, show that three molecules of ligands bind simultaneously to the extremely, large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a, slightly different subset of AcrB residues for binding. The bound ligand, molecules often interact with each other, stabilizing the binding.

Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.

==About this Structure==

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[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Jr., D.E.Koshland.]]

[[Category: Jr., D E.Koshland.]]

[[Category: McDermott, G.]]

[[Category: Nikaido, H.]]

[[Category: Yu, E.W.]]

[[Category: Yu, E W.]]

[[Category: Zgurskaya, H.I.]]

[[Category: Zgurskaya, H I.]]

[[Category: RHQ]]

[[Category: membrane protein]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:00 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Multidrug efflux pumps cause serious problems in cancer chemotherapy and, treatment of bacterial infections. Yet high-resolution structures of, ligand transporter complexes have previously been unavailable. We obtained, x-ray crystallographic structures of the trimeric AcrB pump from, Escherichia coli with four structurally diverse ligands. The structures, show that three molecules of ligands bind simultaneously to the extremely, large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a, slightly different subset of AcrB residues for binding. The bound ligand, molecules often interact with each other, stabilizing the binding.
+Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Jr., D.E.Koshland.]]
+[[Category: Jr., D E.Koshland.]]
 [[Category: McDermott, G.]]
 [[Category: Nikaido, H.]]
-[[Category: Yu, E.W.]]
+[[Category: Yu, E W.]]
-[[Category: Zgurskaya, H.I.]]
+[[Category: Zgurskaya, H I.]]
 [[Category: RHQ]]
 [[Category: membrane protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:37:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:00 2008''