1pag: Difference between revisions

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caption="1pag, resolution 2.8Å" />

'''THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN'''<br />

==Overview==

The pokeweed antiviral protein (PAP), isolated from the leaves of, Phytolacca americana, is one of a family of plant and bacterial, ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases, on rRNA. Here we report the three-dimensional structure of PAP determined, to 2.5 A resolution by X-ray crystallography. After 14 rounds of, refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is, homologous with the A chain of ricin and exhibits a very similar folding, pattern. The positions of key active site residues are also similar. We, also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring, is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3, which is thought to be important in catalysis.

The pokeweed antiviral protein (PAP), isolated from the leaves of Phytolacca americana, is one of a family of plant and bacterial ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases on rRNA. Here we report the three-dimensional structure of PAP determined to 2.5 A resolution by X-ray crystallography. After 14 rounds of refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is homologous with the A chain of ricin and exhibits a very similar folding pattern. The positions of key active site residues are also similar. We also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3 which is thought to be important in catalysis.

==About this Structure==

1PAG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with FMP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].

1PAG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with <scene name='pdbligand=FMP:'>FMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].

==Reference==

Line 13:

[[Category: Phytolacca americana]]

[[Category: Single protein]]

[[Category: Collins, E.J.]]

[[Category: Collins, E J.]]

[[Category: Ernst, S.R.]]

[[Category: Ernst, S R.]]

[[Category: Irvin, J.D.]]

[[Category: Irvin, J D.]]

[[Category: Monzingo, A.F.]]

[[Category: Monzingo, A F.]]

[[Category: Robertus, J.D.]]

[[Category: Robertus, J D.]]

[[Category: FMP]]

[[Category: protein synthesis inhibitor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:38:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:59 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pag.jpg|left|200px]]<br /><applet load="1pag" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pag.jpg|left|200px]]<br /><applet load="1pag" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pag, resolution 2.8&Aring;" />
 '''THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN'''<br />
 ==Overview==
-The pokeweed antiviral protein (PAP), isolated from the leaves of, Phytolacca americana, is one of a family of plant and bacterial, ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases, on rRNA. Here we report the three-dimensional structure of PAP determined, to 2.5 A resolution by X-ray crystallography. After 14 rounds of, refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is, homologous with the A chain of ricin and exhibits a very similar folding, pattern. The positions of key active site residues are also similar. We, also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring, is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3, which is thought to be important in catalysis.
+The pokeweed antiviral protein (PAP), isolated from the leaves of Phytolacca americana, is one of a family of plant and bacterial ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases on rRNA. Here we report the three-dimensional structure of PAP determined to 2.5 A resolution by X-ray crystallography. After 14 rounds of refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is homologous with the A chain of ricin and exhibits a very similar folding pattern. The positions of key active site residues are also similar. We also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3 which is thought to be important in catalysis.
 ==About this Structure==
-PAG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with FMP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].
+PAG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with <scene name='pdbligand=FMP:'>FMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Phytolacca americana]]
 [[Category: Single protein]]
-[[Category: Collins, E.J.]]
+[[Category: Collins, E J.]]
-[[Category: Ernst, S.R.]]
+[[Category: Ernst, S R.]]
-[[Category: Irvin, J.D.]]
+[[Category: Irvin, J D.]]
-[[Category: Monzingo, A.F.]]
+[[Category: Monzingo, A F.]]
-[[Category: Robertus, J.D.]]
+[[Category: Robertus, J D.]]
 [[Category: FMP]]
 [[Category: protein synthesis inhibitor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:38:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:59 2008''