1pny: Difference between revisions

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[[Image:1pny.jpg|left|200px]]<br /><applet load="1pny" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pny.jpg|left|200px]]<br /><applet load="1pny" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pny, resolution 9.5Å" />

'''Crystal Structure of the Wild Type Ribosome from E. Coli, 50S Subunit of 70S Ribosome. THIS FILE, 1PNY, CONTAINS ONLY MOLECULES OF THE 50S RIBOSOMAL SUBUNIT. THE 30S SUBUNIT IS IN THE PDB FILE 1PNX.'''<br />

==Overview==

Protein biosynthesis on the ribosome requires accurate reading of the, genetic code in mRNA. Two conformational rearrangements in the small, ribosomal subunit, a closing of the head and body around the incoming tRNA, and an RNA helical switch near the mRNA decoding site, have been proposed, to select for complementary base-pairing between mRNA codons and tRNA, anticodons. We determined x-ray crystal structures of the WT and a, hyper-accurate variant of the Escherichia coli ribosome at resolutions of, 10 and 9 A, respectively, revealing that formation of the intact 70S, ribosome from its two subunits closes the conformation of the head of the, small subunit independent of mRNA decoding. Moreover, no change in the, conformation of the switch helix is observed in two steps of tRNA, discrimination. These 70S ribosome structures indicate that mRNA decoding, is coupled primarily to movement of the small subunit body, consistent, with previous proposals, whereas closing of the head and the helical, switch may function in other steps of protein synthesis.

Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.

==About this Structure==

1PNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PNY OCA].

1PNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNY OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Cate, J.H.D.]]

[[Category: Cate, J H.D.]]

[[Category: Ridgeway, W.K.]]

[[Category: Ridgeway, W K.]]

[[Category: Santoso, S.]]

[[Category: Seymaner, V.]]

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[[Category: rna-rna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:46:~~36 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:41 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pny.jpg|left|200px]]<br /><applet load="1pny" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pny.jpg|left|200px]]<br /><applet load="1pny" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pny, resolution 9.5&Aring;" />
 '''Crystal Structure of the Wild Type Ribosome from E. Coli, 50S Subunit of 70S Ribosome. THIS FILE, 1PNY, CONTAINS ONLY MOLECULES OF THE 50S RIBOSOMAL SUBUNIT. THE 30S SUBUNIT IS IN THE PDB FILE 1PNX.'''<br />
 ==Overview==
-Protein biosynthesis on the ribosome requires accurate reading of the, genetic code in mRNA. Two conformational rearrangements in the small, ribosomal subunit, a closing of the head and body around the incoming tRNA, and an RNA helical switch near the mRNA decoding site, have been proposed, to select for complementary base-pairing between mRNA codons and tRNA, anticodons. We determined x-ray crystal structures of the WT and a, hyper-accurate variant of the Escherichia coli ribosome at resolutions of, 10 and 9 A, respectively, revealing that formation of the intact 70S, ribosome from its two subunits closes the conformation of the head of the, small subunit independent of mRNA decoding. Moreover, no change in the, conformation of the switch helix is observed in two steps of tRNA, discrimination. These 70S ribosome structures indicate that mRNA decoding, is coupled primarily to movement of the small subunit body, consistent, with previous proposals, whereas closing of the head and the helical, switch may function in other steps of protein synthesis.
+Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.
 ==About this Structure==
-PNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PNY OCA].
+PNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNY OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Cate, J.H.D.]]
+[[Category: Cate, J H.D.]]
-[[Category: Ridgeway, W.K.]]
+[[Category: Ridgeway, W K.]]
 [[Category: Santoso, S.]]
 [[Category: Seymaner, V.]]
@@ Line 25: / Line 25: @@
 [[Category: rna-rna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:46:36 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:41 2008''