Sandbox Reserved 641: Difference between revisions
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::[[Image:glutamatedehydrogenase.jpeg]] | ::[[Image:glutamatedehydrogenase.jpeg]] | ||
Glutamate dehydrogenase is important in nitrogen and glutamate metabolism and energy homeostasis. In the reaction above the forward reaction is essential in converting free ammonia and α-ketoglutarate to glutamate, an amino acid that is used for protein synthesis. The reverse reaction is key reaction that links amino acid metabolism with the Tricarboxylic Acid cycle (TCA cycle). Both reactions utilize nicotinamide nucleotide cofactors: NAD+ when nitrogen is released and NADPH when nitrogen is used. Glutamate dehydrogenase is regulated by cell energy charge. This requires Adenosine triphosphate (ATP) and Guanosine triphosphate (GTP) are positive allosteric effectors for the forward reaction and Adenosine diphosphate (ADP) and Guanosine diphosphate are positive allosteric effectors for the reverse reaction. When the level of ATP is high, conversion of glutamate to α-ketoglurate and other TCA cycle intermediates is limited; when the cellular energy charge is low, glutamate is converted to ammonia and oxidizable TCA cycle intermediates. Glutamate is an important amino acid since it gives an amine group for many transamination reactions, thus, glutamate dehydrogenase is essential in producing this amino acid. | |||
(1) http://www.sciencedirect.com.prox.lib.ncsu.edu/science/article/pii/S0968000408001898 | (1) http://www.sciencedirect.com.prox.lib.ncsu.edu/science/article/pii/S0968000408001898 | ||
