1pvb: Difference between revisions

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[[Image:1pvb.jpg|left|200px]]<br /><applet load="1pvb" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pvb.jpg|left|200px]]<br /><applet load="1pvb" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pvb, resolution 1.75Å" />

'''X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN'''<br />

==Overview==

A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in, presence of EDTA at pH 8.0. The crystals are orthorhombic, space group, P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data, were collected to 1.75 A. The structure was solved by molecular, replacement and refined to R = 0.168 for 7774 observed reflections [I>/=, 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the, previously reported structures, the main differences concern the, conformation of the N-terminal residues and the packing in the unit cell.

A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in presence of EDTA at pH 8.0. The crystals are orthorhombic, space group P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data were collected to 1.75 A. The structure was solved by molecular replacement and refined to R = 0.168 for 7774 observed reflections [I>/= 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the previously reported structures, the main differences concern the conformation of the N-terminal residues and the packing in the unit cell.

==About this Structure==

1PVB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius] with CA, NH4 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA].

1PVB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NH4:'>NH4</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA].

==Reference==

Line 13:

[[Category: Esox lucius]]

[[Category: Single protein]]

[[Category: Declercq, J.P.]]

[[Category: Declercq, J P.]]

[[Category: Parello, J.]]

[[Category: Tinant, B.]]

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[[Category: calcium binding protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:09:~~10 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:53 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pvb.jpg|left|200px]]<br /><applet load="1pvb" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pvb.jpg|left|200px]]<br /><applet load="1pvb" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pvb, resolution 1.75&Aring;" />
 '''X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN'''<br />
 ==Overview==
-A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in, presence of EDTA at pH 8.0. The crystals are orthorhombic, space group, P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data, were collected to 1.75 A. The structure was solved by molecular, replacement and refined to R = 0.168 for 7774 observed reflections [I&gt;/=, 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the, previously reported structures, the main differences concern the, conformation of the N-terminal residues and the packing in the unit cell.
+A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in presence of EDTA at pH 8.0. The crystals are orthorhombic, space group P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data were collected to 1.75 A. The structure was solved by molecular replacement and refined to R = 0.168 for 7774 observed reflections [I&gt;/= 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the previously reported structures, the main differences concern the conformation of the N-terminal residues and the packing in the unit cell.
 ==About this Structure==
-PVB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius] with CA, NH4 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA].
+PVB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NH4:'>NH4</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Esox lucius]]
 [[Category: Single protein]]
-[[Category: Declercq, J.P.]]
+[[Category: Declercq, J P.]]
 [[Category: Parello, J.]]
 [[Category: Tinant, B.]]
@@ Line 21: / Line 21: @@
 [[Category: calcium binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:09:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:53 2008''