1pvs: Difference between revisions
New page: left|200px<br /><applet load="1pvs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pvs, resolution 2.40Å" /> '''3-methyladenine Glco... |
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[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size=" | [[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1pvs, resolution 2.40Å" /> | caption="1pvs, resolution 2.40Å" /> | ||
'''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex'''<br /> | '''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex'''<br /> | ||
==Overview== | ==Overview== | ||
Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II | Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity. | ||
==About this Structure== | ==About this Structure== | ||
1PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 7HP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http:// | 1PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=7HP:'>7HP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: reaction-product complex]] | [[Category: reaction-product complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:07 2008'' | ||
Revision as of 12:33, 21 February 2008
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3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex
Overview
Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.
About this Structure
1PVS is a Single protein structure of sequence from Escherichia coli with 7HP as ligand. Active as DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 Full crystallographic information is available from OCA.
Reference
3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:12009927
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