1q5z: Difference between revisions

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[[Image:1q5z.jpg|left|200px]]<br /><applet load="1q5z" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1q5z.jpg|left|200px]]<br /><applet load="1q5z" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1q5z, resolution 1.8Å" />

'''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)'''<br />

==Overview==

Like many bacterial pathogens, Salmonella spp. use a type III secretion, system to inject virulence proteins into host cells. The Salmonella, invasion protein A (SipA) binds host actin, enhances its polymerization, near adherent extracellular bacteria, and contributes to cytoskeletal, rearrangements that internalize the pathogen. By combining x-ray, crystallography of SipA with electron microscopy and image analysis of, SipA-actin filaments, we show that SipA functions as a "molecular staple,", in which a globular domain and two nonglobular "arms" mechanically, stabilize the filament by tethering actin subunits in opposing strands., Deletion analysis of the tethering arms provides strong support for this, model.

Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.

==About this Structure==

1Q5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].

1Q5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].

==Reference==

Line 13:

[[Category: Salmonella typhimurium]]

[[Category: Single protein]]

[[Category: Egelman, E.H.]]

[[Category: Egelman, E H.]]

[[Category: Galkin, V.E.]]

[[Category: Galkin, V E.]]

[[Category: Lilic, M.]]

[[Category: Orlova, A.]]

[[Category: Stebbins, C.E.]]

[[Category: Stebbins, C E.]]

[[Category: VanLoock, M.S.]]

[[Category: VanLoock, M S.]]

[[Category: cell invasion]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:24:~~43 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:12 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1q5z.jpg|left|200px]]<br /><applet load="1q5z" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1q5z.jpg|left|200px]]<br /><applet load="1q5z" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1q5z, resolution 1.8&Aring;" />
 '''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)'''<br />
 ==Overview==
-Like many bacterial pathogens, Salmonella spp. use a type III secretion, system to inject virulence proteins into host cells. The Salmonella, invasion protein A (SipA) binds host actin, enhances its polymerization, near adherent extracellular bacteria, and contributes to cytoskeletal, rearrangements that internalize the pathogen. By combining x-ray, crystallography of SipA with electron microscopy and image analysis of, SipA-actin filaments, we show that SipA functions as a "molecular staple,", in which a globular domain and two nonglobular "arms" mechanically, stabilize the filament by tethering actin subunits in opposing strands., Deletion analysis of the tethering arms provides strong support for this, model.
+Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.
 ==About this Structure==
-Q5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].
+Q5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Salmonella typhimurium]]
 [[Category: Single protein]]
-[[Category: Egelman, E.H.]]
+[[Category: Egelman, E H.]]
-[[Category: Galkin, V.E.]]
+[[Category: Galkin, V E.]]
 [[Category: Lilic, M.]]
 [[Category: Orlova, A.]]
-[[Category: Stebbins, C.E.]]
+[[Category: Stebbins, C E.]]
-[[Category: VanLoock, M.S.]]
+[[Category: VanLoock, M S.]]
 [[Category: cell invasion]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:24:43 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:12 2008''