1qcs: Difference between revisions

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[[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1qcs, resolution 1.90Å" />

'''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br />

==Overview==

N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential, for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles, cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans, complex formation. We have determined the crystal structure of the, N-terminal domain of NSF (N) to 1.9 A resolution. N contains two, subdomains which form a groove that is a likely SNAP interaction site., Unexpectedly, both N subdomains are structurally similar to domains in, EF-Tu. Based on this similarity, we propose a model for a large, conformational change in NSF that drives SNARE complex disassembly.

N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.

==About this Structure==

1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA].

1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA].

==Reference==

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[[Category: Cricetulus griseus]]

[[Category: Single protein]]

[[Category: Brunger, A.T.]]

[[Category: Brunger, A T.]]

[[Category: Jahn, R.]]

[[Category: Yu, R.C.]]

[[Category: Yu, R C.]]

[[Category: SO4]]

[[Category: double-psi beta barrel alpha beta roll]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:34:~~52 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:20 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qcs, resolution 1.90&Aring;" />
 '''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br />
 ==Overview==
-N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential, for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles, cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans, complex formation. We have determined the crystal structure of the, N-terminal domain of NSF (N) to 1.9 A resolution. N contains two, subdomains which form a groove that is a likely SNAP interaction site., Unexpectedly, both N subdomains are structurally similar to domains in, EF-Tu. Based on this similarity, we propose a model for a large, conformational change in NSF that drives SNARE complex disassembly.
+N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.
 ==About this Structure==
-QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA].
+QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Cricetulus griseus]]
 [[Category: Single protein]]
-[[Category: Brunger, A.T.]]
+[[Category: Brunger, A T.]]
 [[Category: Jahn, R.]]
-[[Category: Yu, R.C.]]
+[[Category: Yu, R C.]]
 [[Category: SO4]]
 [[Category: double-psi beta barrel alpha beta roll]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:34:52 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:20 2008''