1r30: Difference between revisions
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New page: left|200px<br /><applet load="1r30" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r30, resolution 3.40Å" /> '''The Crystal Structur... |
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[[Image:1r30.gif|left|200px]]<br /><applet load="1r30" size=" | [[Image:1r30.gif|left|200px]]<br /><applet load="1r30" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1r30, resolution 3.40Å" /> | caption="1r30, resolution 3.40Å" /> | ||
'''The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme'''<br /> | '''The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of biotin synthase from Escherichia coli in complex | The crystal structure of biotin synthase from Escherichia coli in complex with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4 angstrom resolution. This structure addresses how "AdoMet radical" or "radical SAM" enzymes use Fe4S4 clusters and S-adenosyl-L-methionine to generate organic radicals. Biotin synthase catalyzes the radical-mediated insertion of sulfur into dethiobiotin to form biotin. The structure places the substrates between the Fe4S4 cluster, essential for radical generation, and the Fe2S2 cluster, postulated to be the source of sulfur, with both clusters in unprecedented coordination environments. | ||
==About this Structure== | ==About this Structure== | ||
1R30 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM, SF4, FES, DTB and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Biotin_synthase Biotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.6 2.8.1.6] Full crystallographic information is available from [http:// | 1R30 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SAM:'>SAM</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=DTB:'>DTB</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Biotin_synthase Biotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.6 2.8.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R30 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Berkovitch, F.]] | [[Category: Berkovitch, F.]] | ||
[[Category: Drennan, C | [[Category: Drennan, C L.]] | ||
[[Category: Jarrett, J | [[Category: Jarrett, J T.]] | ||
[[Category: Nicolet, Y.]] | [[Category: Nicolet, Y.]] | ||
[[Category: Wan, J | [[Category: Wan, J T.]] | ||
[[Category: DTB]] | [[Category: DTB]] | ||
[[Category: FES]] | [[Category: FES]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:30 2008'' | ||
