1rw3: Difference between revisions

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[[Image:1rw3.gif|left|200px]]<br /><applet load="1rw3" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1rw3.gif|left|200px]]<br /><applet load="1rw3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1rw3, resolution 3.0Å" />

'''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''<br />

==Overview==

Reverse transcriptases (RTs) are multidomain enzymes of variable, architecture that couple both RNA- and DNA-directed DNA polymerase, activities with an RNase H activity specific for an RNA:DNA hybrid in, order to replicate the single-stranded RNA genome of the retrovirus., Previous structural work has been reported for the heterodimeric HIV-1 and, HIV-2 RTs. We now report the first crystal structure of the full-length, Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure, reveals a clamp-shaped molecule resulting from the relative positions of, the thumb, connection, and RNase H domains that is strikingly different, from the HIV-1 RT and provides the first example of a monomeric reverse, transcriptase. A comparative analysis with related DNA polymerases, suggests a unique trajectory for the template-primer exiting the, polymerase active site and provides insights regarding processive DNA, synthesis by MMLV RT.

Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.

==About this Structure==

1RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].

1RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Das, D.]]

[[Category: Georgiadis, M.M.]]

[[Category: Georgiadis, M M.]]

[[Category: reverse transcriptase]]

[[Category: rna and dna dependent dna polymerase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 01:54:~~02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:08 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1rw3.gif|left|200px]]<br /><applet load="1rw3" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1rw3.gif|left|200px]]<br /><applet load="1rw3" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1rw3, resolution 3.0&Aring;" />
 '''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''<br />
 ==Overview==
-Reverse transcriptases (RTs) are multidomain enzymes of variable, architecture that couple both RNA- and DNA-directed DNA polymerase, activities with an RNase H activity specific for an RNA:DNA hybrid in, order to replicate the single-stranded RNA genome of the retrovirus., Previous structural work has been reported for the heterodimeric HIV-1 and, HIV-2 RTs. We now report the first crystal structure of the full-length, Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure, reveals a clamp-shaped molecule resulting from the relative positions of, the thumb, connection, and RNase H domains that is strikingly different, from the HIV-1 RT and provides the first example of a monomeric reverse, transcriptase. A comparative analysis with related DNA polymerases, suggests a unique trajectory for the template-primer exiting the, polymerase active site and provides insights regarding processive DNA, synthesis by MMLV RT.
+Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.
 ==About this Structure==
-RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
+RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Das, D.]]
-[[Category: Georgiadis, M.M.]]
+[[Category: Georgiadis, M M.]]
 [[Category: reverse transcriptase]]
 [[Category: rna and dna dependent dna polymerase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:54:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:08 2008''