1s1i: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1s1i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s1i" /> '''Structure of the ribosomal 80S-eEF2-sordarin... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1s1i.gif|left|200px]]<br /><applet load="1s1i" size=" | [[Image:1s1i.gif|left|200px]]<br /><applet load="1s1i" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1s1i" /> | caption="1s1i" /> | ||
'''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.'''<br /> | '''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.'''<br /> | ||
==Overview== | ==Overview== | ||
An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the | An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction. | ||
==About this Structure== | ==About this Structure== | ||
1S1I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | 1S1I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1I OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Andersen, G | [[Category: Andersen, G R.]] | ||
[[Category: Ballesta, J | [[Category: Ballesta, J P.G.]] | ||
[[Category: Beckmann, R.]] | [[Category: Beckmann, R.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Gomez-Lorenzo, M | [[Category: Gomez-Lorenzo, M G.]] | ||
[[Category: Grassucci, R | [[Category: Grassucci, R A.]] | ||
[[Category: Jorgensen, R.]] | [[Category: Jorgensen, R.]] | ||
[[Category: Penczek, P | [[Category: Penczek, P A.]] | ||
[[Category: Spahn, C | [[Category: Spahn, C M.]] | ||
[[Category: 60s ribosomal subunit]] | [[Category: 60s ribosomal subunit]] | ||
[[Category: 80s ribosome]] | [[Category: 80s ribosome]] | ||
| Line 29: | Line 29: | ||
[[Category: trna translocation]] | [[Category: trna translocation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:52 2008'' | ||
