1s9b: Difference between revisions

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[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1s9b, resolution 2.81Å" />

'''Crystal Structure Analysis of the B-DNA GAATTCG'''<br />

==Overview==

DNA oligonucleotides can be used in order to assemble highly structured, materials. Oligonucleotides with sticky ends can form long linear, structures, whereas branching is required to form two- and, three-dimensional nanostructures. In this paper, we show that when Ni(2+), is attached to the N7 atom of guanine, it can also act as a branching, point. Thus, we have found that the heptanucleotide d(GAATTCG) can, assemble into long linear duplex structures, which cross in space to, generate a cubic structure. The three-dimensional arrays are stabilized by, phosphate-Ni(2+)-guanine interactions. For the first time, the, crystallization of a B form DNA oligonucleotide in a cubic system is, reported, space group I23. Large solvent cavities are found among the DNA, duplexes.

DNA oligonucleotides can be used in order to assemble highly structured materials. Oligonucleotides with sticky ends can form long linear structures, whereas branching is required to form two- and three-dimensional nanostructures. In this paper, we show that when Ni(2+) is attached to the N7 atom of guanine, it can also act as a branching point. Thus, we have found that the heptanucleotide d(GAATTCG) can assemble into long linear duplex structures, which cross in space to generate a cubic structure. The three-dimensional arrays are stabilized by phosphate-Ni(2+)-guanine interactions. For the first time, the crystallization of a B form DNA oligonucleotide in a cubic system is reported, space group I23. Large solvent cavities are found among the DNA duplexes.

==About this Structure==

1S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].

1S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Gouyette, C.]]

[[Category: Subirana, J.A.]]

[[Category: Subirana, J A.]]

[[Category: Uson, I.]]

[[Category: Valls, N.]]

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[[Category: nickel]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:10 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1s9b, resolution 2.81&Aring;" />
 '''Crystal Structure Analysis of the B-DNA GAATTCG'''<br />
 ==Overview==
-DNA oligonucleotides can be used in order to assemble highly structured, materials. Oligonucleotides with sticky ends can form long linear, structures, whereas branching is required to form two- and, three-dimensional nanostructures. In this paper, we show that when Ni(2+), is attached to the N7 atom of guanine, it can also act as a branching, point. Thus, we have found that the heptanucleotide d(GAATTCG) can, assemble into long linear duplex structures, which cross in space to, generate a cubic structure. The three-dimensional arrays are stabilized by, phosphate-Ni(2+)-guanine interactions. For the first time, the, crystallization of a B form DNA oligonucleotide in a cubic system is, reported, space group I23. Large solvent cavities are found among the DNA, duplexes.
+DNA oligonucleotides can be used in order to assemble highly structured materials. Oligonucleotides with sticky ends can form long linear structures, whereas branching is required to form two- and three-dimensional nanostructures. In this paper, we show that when Ni(2+) is attached to the N7 atom of guanine, it can also act as a branching point. Thus, we have found that the heptanucleotide d(GAATTCG) can assemble into long linear duplex structures, which cross in space to generate a cubic structure. The three-dimensional arrays are stabilized by phosphate-Ni(2+)-guanine interactions. For the first time, the crystallization of a B form DNA oligonucleotide in a cubic system is reported, space group I23. Large solvent cavities are found among the DNA duplexes.
 ==About this Structure==
-S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].
+S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Gouyette, C.]]
-[[Category: Subirana, J.A.]]
+[[Category: Subirana, J A.]]
 [[Category: Uson, I.]]
 [[Category: Valls, N.]]
@@ Line 21: / Line 21: @@
 [[Category: nickel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:06:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:10 2008''