1shz: Difference between revisions

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[[Image:1shz.gif|left|200px]]<br />

[[Image:1shz.gif|left|200px]]<br /><applet load="1shz" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1shz" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1shz, resolution 2.85Å" />

'''Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera'''<br />

==Overview==

p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is, also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric, Galpha subunits. The GAP function of p115RhoGEF resides within the, N-terminal region of p115RhoGEF (the rgRGS domain), which includes a, module that is structurally similar to RGS (regulators of G-protein, signaling) domains. We present here the crystal structure of the rgRGS, domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1., Two distinct surfaces of rgRGS interact with Galpha. The N-terminal, betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate, contacts with the catalytic site of Galpha. The interface between the RGS, module of rgRGS and Galpha is similar to that of a Galpha-effector, complex, suggesting a role for the rgRGS domain in the stimulation of the, GEF activity of p115RhoGEF by Galpha13.

p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13.

==Disease==

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==About this Structure==

1SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with MG, ALF and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SHZ OCA].

1SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHZ OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Chen, Z.]]

[[Category: Singer, W.D.]]

[[Category: Singer, W D.]]

[[Category: Sprang, S.R.]]

[[Category: Sprang, S R.]]

[[Category: Sternweis, P.C.]]

[[Category: Sternweis, P C.]]

[[Category: ALF]]

[[Category: GDP]]

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[[Category: signal transduction]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 19~~:14:~~02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:41 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1shz.gif|left|200px]]<br />
+[[Image:1shz.gif|left|200px]]<br /><applet load="1shz" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1shz" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1shz, resolution 2.85&Aring;" />
 '''Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera'''<br />
 ==Overview==
-p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is, also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric, Galpha subunits. The GAP function of p115RhoGEF resides within the, N-terminal region of p115RhoGEF (the rgRGS domain), which includes a, module that is structurally similar to RGS (regulators of G-protein, signaling) domains. We present here the crystal structure of the rgRGS, domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1., Two distinct surfaces of rgRGS interact with Galpha. The N-terminal, betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate, contacts with the catalytic site of Galpha. The interface between the RGS, module of rgRGS and Galpha is similar to that of a Galpha-effector, complex, suggesting a role for the rgRGS domain in the stimulation of the, GEF activity of p115RhoGEF by Galpha13.
+p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with MG, ALF and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SHZ OCA].
+SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHZ OCA].
 ==Reference==
@@ Line 19: / Line 18: @@
 [[Category: Protein complex]]
 [[Category: Chen, Z.]]
-[[Category: Singer, W.D.]]
+[[Category: Singer, W D.]]
-[[Category: Sprang, S.R.]]
+[[Category: Sprang, S R.]]
-[[Category: Sternweis, P.C.]]
+[[Category: Sternweis, P C.]]
 [[Category: ALF]]
 [[Category: GDP]]
@@ Line 28: / Line 27: @@
 [[Category: signal transduction]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:14:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:41 2008''