1siz: Difference between revisions

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[[Image:1siz.gif|left|200px]]<br /><applet load="1siz" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1siz.gif|left|200px]]<br /><applet load="1siz" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1siz, resolution 2.25Å" />

'''Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus'''<br />

==Overview==

The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic, archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from, a crystal belonging to space group P2(1) with two molecules in the, asymmetric unit. The structure has been solved with molecular replacement, by use of the ferredoxin from Thermotoga maritima. The fold is similar to, that of related monocluster ferredoxins and contains two double-stranded, antiparallel beta-sheets and two alpha-helices. The hydrophobic, interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to, the longer alpha-helix. The structure contains a double-conformation, disulfide bond existing in a left-handed and a right-handed spiral, conformation. The crystal packing reveals a beta-sheet interaction, which, supports the suggestion that P. furiosus ferredoxin is a functional dimer., The extraordinary thermostability of P. furiosus ferredoxin is further, discussed.

The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from a crystal belonging to space group P2(1) with two molecules in the asymmetric unit. The structure has been solved with molecular replacement by use of the ferredoxin from Thermotoga maritima. The fold is similar to that of related monocluster ferredoxins and contains two double-stranded antiparallel beta-sheets and two alpha-helices. The hydrophobic interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to the longer alpha-helix. The structure contains a double-conformation disulfide bond existing in a left-handed and a right-handed spiral conformation. The crystal packing reveals a beta-sheet interaction, which supports the suggestion that P. furiosus ferredoxin is a functional dimer. The extraordinary thermostability of P. furiosus ferredoxin is further discussed.

==About this Structure==

1SIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with 3CO and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SIZ OCA].

1SIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=3CO:'>3CO</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIZ OCA].

==Reference==

Line 13:

[[Category: Pyrococcus furiosus]]

[[Category: Single protein]]

[[Category: Christensen, H.E.M.]]

[[Category: Christensen, H E.M.]]

[[Category: Harris, P.]]

[[Category: Nielsen, M.S.]]

[[Category: Nielsen, M S.]]

[[Category: 3CO]]

[[Category: F3S]]

Line 22:

[[Category: thermostability]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:58 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1siz.gif|left|200px]]<br /><applet load="1siz" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1siz.gif|left|200px]]<br /><applet load="1siz" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1siz, resolution 2.25&Aring;" />
 '''Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus'''<br />
 ==Overview==
-The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic, archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from, a crystal belonging to space group P2(1) with two molecules in the, asymmetric unit. The structure has been solved with molecular replacement, by use of the ferredoxin from Thermotoga maritima. The fold is similar to, that of related monocluster ferredoxins and contains two double-stranded, antiparallel beta-sheets and two alpha-helices. The hydrophobic, interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to, the longer alpha-helix. The structure contains a double-conformation, disulfide bond existing in a left-handed and a right-handed spiral, conformation. The crystal packing reveals a beta-sheet interaction, which, supports the suggestion that P. furiosus ferredoxin is a functional dimer., The extraordinary thermostability of P. furiosus ferredoxin is further, discussed.
+The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from a crystal belonging to space group P2(1) with two molecules in the asymmetric unit. The structure has been solved with molecular replacement by use of the ferredoxin from Thermotoga maritima. The fold is similar to that of related monocluster ferredoxins and contains two double-stranded antiparallel beta-sheets and two alpha-helices. The hydrophobic interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to the longer alpha-helix. The structure contains a double-conformation disulfide bond existing in a left-handed and a right-handed spiral conformation. The crystal packing reveals a beta-sheet interaction, which supports the suggestion that P. furiosus ferredoxin is a functional dimer. The extraordinary thermostability of P. furiosus ferredoxin is further discussed.
 ==About this Structure==
-SIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with 3CO and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SIZ OCA].
+SIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=3CO:'>3CO</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIZ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Pyrococcus furiosus]]
 [[Category: Single protein]]
-[[Category: Christensen, H.E.M.]]
+[[Category: Christensen, H E.M.]]
 [[Category: Harris, P.]]
-[[Category: Nielsen, M.S.]]
+[[Category: Nielsen, M S.]]
 [[Category: 3CO]]
 [[Category: F3S]]
@@ Line 22: / Line 22: @@
 [[Category: thermostability]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:24:50 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:58 2008''