1sxl: Difference between revisions

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[[Image:1sxl.gif|left|200px]]<br /><applet load="1sxl" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1sxl.gif|left|200px]]<br /><applet load="1sxl" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1sxl" />

'''RESONANCE ASSIGNMENTS AND SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN OF SEX-LETHAL DETERMINED BY MULTIDIMENSIONAL HETERONUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''<br />

==Overview==

The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual, differentiation and dosage compensation in Drosophila. This regulatory, activity is a consequence of the ability of Sxl to bind uridine-rich RNA, tracts involved in pre-mRNA splicing. Sxl contains two RNP consensus-type, RNA-binding domains (RBDs). A structural study of a portion of Sxl (amino, acids 199-294) containing the second RNA-binding domain (RBD-2) using, multidimensional heteronuclear NMR is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have been obtained from 15N- and, 13C/15N-uniformly labeled protein. These assignments were used to analyze, 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY spectra which, produced 494 total and 169 long-range NOE-derived distance restraints., Along with 41 backbone dihedral restraints, these distance restraints were, employed to generate an intermediate-resolution family of calculated, structures, which exhibits the beta alpha beta-beta alpha beta tertiary, fold found in other RBD-containing proteins. The RMSD to the average, structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the RMSD for backbone atoms involved in secondary structure is 0.76, +/- 0.14 A. A capping box [Harper, E.T., & Rose, G.D. (1993) Biochemistry, 32, 7605-7609] was identified at the N-terminus of the first helix and has, been characterized by short- and medium-range NOEs. Finally, significant, structural similarities and differences between Sxl RBD-2 and other, RBD-containing proteins are discussed.

The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual differentiation and dosage compensation in Drosophila. This regulatory activity is a consequence of the ability of Sxl to bind uridine-rich RNA tracts involved in pre-mRNA splicing. Sxl contains two RNP consensus-type RNA-binding domains (RBDs). A structural study of a portion of Sxl (amino acids 199-294) containing the second RNA-binding domain (RBD-2) using multidimensional heteronuclear NMR is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have been obtained from 15N- and 13C/15N-uniformly labeled protein. These assignments were used to analyze 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY spectra which produced 494 total and 169 long-range NOE-derived distance restraints. Along with 41 backbone dihedral restraints, these distance restraints were employed to generate an intermediate-resolution family of calculated structures, which exhibits the beta alpha beta-beta alpha beta tertiary fold found in other RBD-containing proteins. The RMSD to the average structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the RMSD for backbone atoms involved in secondary structure is 0.76 +/- 0.14 A. A capping box [Harper, E.T., & Rose, G.D. (1993) Biochemistry 32, 7605-7609] was identified at the N-terminus of the first helix and has been characterized by short- and medium-range NOEs. Finally, significant structural similarities and differences between Sxl RBD-2 and other RBD-containing proteins are discussed.

==About this Structure==

1SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SXL OCA].

1SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXL OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Kanaar, R.]]

[[Category: Lee, A.L.]]

[[Category: Lee, A L.]]

[[Category: Rio, D.C.]]

[[Category: Rio, D C.]]

[[Category: Wemmer, D.E.]]

[[Category: Wemmer, D E.]]

[[Category: rna-binding protein]]

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@@ Line 1: / Line 1: @@
-[[Image:1sxl.gif|left|200px]]<br /><applet load="1sxl" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1sxl.gif|left|200px]]<br /><applet load="1sxl" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1sxl" />
 '''RESONANCE ASSIGNMENTS AND SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN OF SEX-LETHAL DETERMINED BY MULTIDIMENSIONAL HETERONUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''<br />
 ==Overview==
-The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual, differentiation and dosage compensation in Drosophila. This regulatory, activity is a consequence of the ability of Sxl to bind uridine-rich RNA, tracts involved in pre-mRNA splicing. Sxl contains two RNP consensus-type, RNA-binding domains (RBDs). A structural study of a portion of Sxl (amino, acids 199-294) containing the second RNA-binding domain (RBD-2) using, multidimensional heteronuclear NMR is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have been obtained from 15N- and, 13C/15N-uniformly labeled protein. These assignments were used to analyze, 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY spectra which, produced 494 total and 169 long-range NOE-derived distance restraints., Along with 41 backbone dihedral restraints, these distance restraints were, employed to generate an intermediate-resolution family of calculated, structures, which exhibits the beta alpha beta-beta alpha beta tertiary, fold found in other RBD-containing proteins. The RMSD to the average, structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the RMSD for backbone atoms involved in secondary structure is 0.76, +/- 0.14 A. A capping box [Harper, E.T., &amp; Rose, G.D. (1993) Biochemistry, 32, 7605-7609] was identified at the N-terminus of the first helix and has, been characterized by short- and medium-range NOEs. Finally, significant, structural similarities and differences between Sxl RBD-2 and other, RBD-containing proteins are discussed.
+The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual differentiation and dosage compensation in Drosophila. This regulatory activity is a consequence of the ability of Sxl to bind uridine-rich RNA tracts involved in pre-mRNA splicing. Sxl contains two RNP consensus-type RNA-binding domains (RBDs). A structural study of a portion of Sxl (amino acids 199-294) containing the second RNA-binding domain (RBD-2) using multidimensional heteronuclear NMR is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have been obtained from 15N- and 13C/15N-uniformly labeled protein. These assignments were used to analyze 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY spectra which produced 494 total and 169 long-range NOE-derived distance restraints. Along with 41 backbone dihedral restraints, these distance restraints were employed to generate an intermediate-resolution family of calculated structures, which exhibits the beta alpha beta-beta alpha beta tertiary fold found in other RBD-containing proteins. The RMSD to the average structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the RMSD for backbone atoms involved in secondary structure is 0.76 +/- 0.14 A. A capping box [Harper, E.T., &amp; Rose, G.D. (1993) Biochemistry 32, 7605-7609] was identified at the N-terminus of the first helix and has been characterized by short- and medium-range NOEs. Finally, significant structural similarities and differences between Sxl RBD-2 and other RBD-containing proteins are discussed.
 ==About this Structure==
-SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SXL OCA].
+SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXL OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Kanaar, R.]]
-[[Category: Lee, A.L.]]
+[[Category: Lee, A L.]]
-[[Category: Rio, D.C.]]
+[[Category: Rio, D C.]]
-[[Category: Wemmer, D.E.]]
+[[Category: Wemmer, D E.]]
 [[Category: rna-binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:49:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:06:38 2008''