Sandbox Reserved 712: Difference between revisions
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=== Enzymatic analysis of recombinant PRs === | === Enzymatic analysis of recombinant PRs === | ||
Samples | Samples PR<sub>drv1</sub> to PR<sub>drv6</sub> have been cloned and expressed in ''E. coli''. Then they were purified anch characterized in vitro by monitoring cleavage of a chromogenic peptide substrate in the presence and absence of specific PIs. <ref name="Molecular" /> | ||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738195/table/t5/ Enzyme characteristics of PR variants analyzed in this study] | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738195/table/t5/ Enzyme characteristics of PR variants analyzed in this study] | ||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738195/table/t6/ K<sub>i</sub> values for the inhibitors of PR mutants] | |||
=== X-ray strucure analysis of PRdrv1 and PRdrv5 === | === X-ray strucure analysis of PRdrv1 and PRdrv5 === | ||
