Sandbox Reserved 712: Difference between revisions

From Proteopedia
Jump to navigationJump to search
Line 26: Line 26:
=== Enzymatic analysis of recombinant PRs ===
=== Enzymatic analysis of recombinant PRs ===


Samples PR<sub>drv1</sub> to PR<sub>drv6</sub> have been cloned and expressed in ''E. coli''. Then they were purified anch characterized in vitro by monitoring cleavage of a chromogenic peptide substrate in the presence and absence of specific PIs. <ref name="Molecular" />
Samples PR<sub>DRV1</sub> to PR<sub>DRV6</sub> have been cloned and expressed in ''E. coli''. Then they were purified anch characterized in vitro by monitoring cleavage of a chromogenic peptide substrate in the presence and absence of specific PIs. <ref name="Molecular" />


[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738195/table/t5/ Enzyme characteristics of PR variants analyzed in  this study]
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738195/table/t5/ Enzyme characteristics of PR variants analyzed in  this study]