Sandbox Reserved 712: Difference between revisions

Line 56:

Surface residues <scene name='Sandbox_Reserved_712/R45/1'>R45</scene> and <scene name='Sandbox_Reserved_712/R55/1'>R55</scene> have disordered side chains, but the other amino acid residue changes could be modeled into well-defined electron density maps.

PRDRV5 contains darunavir mutations V82T and I84V (see Fig.2, Part B, indicated in bold print) that are directly involved in substrate-darunavir-interactions (change of S2/S2' subsites).

PRDRV5 contains darunavir mutations <scene name='Sandbox_Reserved_712/V82t/1'>V82T</scene> and

<scene name='Sandbox_Reserved_712/I84v/2'>I84V</scene> (see Fig.2, Part B, indicated in bold print) that are directly involved in substrate-darunavir-interactions (change of S2/S2' subsites).

The other 18 mutations are outside the binding cleft, but some are still in direct contact with the binding residues (e.g. L10I, K20M, L33F, I54L/V and L90M).

The other 18 mutations are outside the binding cleft, but some are still in direct contact with the binding residues (e.g. <scene name='Sandbox_Reserved_712/L10i/1'>L10I</scene>, K20M, <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/I54v/1'>I54L/V</scene> and <scene name='Sandbox_Reserved_712/L90m/1'>L90M</scene>).

Mutations L33F, M36L, N37T, P39S, K45R, M46I, I54V and K55R cause structural changes in the flap region and the flap hinge.

Mutations <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/M36l/1'>M36L</scene>, <scene name='Sandbox_Reserved_712/N37t/1'>N37T</scene>,

<scene name='Sandbox_Reserved_712/P39s/1'>P39S</scene>, <scene name='Sandbox_Reserved_712/K45r/1'>K45R</scene>, M46I, <scene name='Sandbox_Reserved_712/I54v/1'>I54V</scene> and <scene name='Sandbox_Reserved_712/K55r/1'>K55R</scene> cause structural changes in the flap region and the flap hinge.

The pictures on the right (Fig.3 and Fig.4) show the regions (indicated in blue) that undergo structural changes caused by the mutations.

To see the full images, with changes in PRDRV1 and comparative structure of wild-type PRDRV1 and PRDRV5 follow the links:

Sandbox Reserved 712: Difference between revisions

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@@ Line 56: / Line 56: @@
 Surface residues <scene name='Sandbox_Reserved_712/R45/1'>R45</scene> and <scene name='Sandbox_Reserved_712/R55/1'>R55</scene> have disordered side chains, but the other amino acid residue changes could be modeled into well-defined electron density maps.
-PR<sub>DRV5</sub> contains darunavir mutations V82T and I84V (see Fig.2, Part B, indicated in bold print) that are directly involved in substrate-darunavir-interactions (change of S2/S2' subsites).
+PR<sub>DRV5</sub> contains darunavir mutations <scene name='Sandbox_Reserved_712/V82t/1'>V82T</scene> and
+<scene name='Sandbox_Reserved_712/I84v/2'>I84V</scene> (see Fig.2, Part B, indicated in bold print) that are directly involved in substrate-darunavir-interactions (change of S2/S2' subsites).
-The other 18 mutations are outside the binding cleft, but some are still in direct contact with the binding residues (e.g. L10I, K20M, L33F, I54L/V and L90M).
+The other 18 mutations are outside the binding cleft, but some are still in direct contact with the binding residues (e.g. <scene name='Sandbox_Reserved_712/L10i/1'>L10I</scene>, K20M, <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/I54v/1'>I54L/V</scene> and <scene name='Sandbox_Reserved_712/L90m/1'>L90M</scene>).
-Mutations L33F, M36L, N37T, P39S, K45R, M46I, I54V and K55R cause structural changes in the flap region and the flap hinge.
+Mutations <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/M36l/1'>M36L</scene>, <scene name='Sandbox_Reserved_712/N37t/1'>N37T</scene>,
+<scene name='Sandbox_Reserved_712/P39s/1'>P39S</scene>, <scene name='Sandbox_Reserved_712/K45r/1'>K45R</scene>, M46I, <scene name='Sandbox_Reserved_712/I54v/1'>I54V</scene> and <scene name='Sandbox_Reserved_712/K55r/1'>K55R</scene> cause structural changes in the flap region and the flap hinge.
 The pictures on the right (Fig.3 and Fig.4) show the regions (indicated in blue) that undergo structural changes caused by the mutations.
 To see the full images, with changes in PR<sub>DRV1</sub> and comparative structure of wild-type PR<sub>DRV1</sub> and PR<sub>DRV5</sub> follow the links: