Sandbox Reserved 717: Difference between revisions

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To finish, the phage DNA single-stranded is injected into the bacterial cytoplasm through the tail tube.

== '''~~Role~~ of gp12''' ==

== '''Presentation of gp12''' ==

During the first step of the lytic cycle, the short tail fibers gp12 ~~is attached~~ to the host cell.

During the first step of the lytic cycle, the short tail fibers gp12 attach the virion to the host cell. Thanks to a three-dimensional cryoelectron microscopy, a reconstruction of the baseplate was determined to a resolution of 12 Angstrom. It has shown that the total length of the gp12 density is inferior at 340 Å.

~~A monomer of gp12 has a mass of 55.3 kDa. Gp12 can be divided into two fragments with different masses. Each short tail is composed of three repetitions of the monomer~~. Thanks to a three-dimensional cryoelectron microscopy, a reconstruction of the baseplate was determined to a resolution of 12 A.

This technique was also a way to determine the interactions between the different proteins and their localization. Thus, interactions of the short tail fibers with each other and with the gp11 maintain the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 and gp11 is essential for the attachment of gp12 to the baseplate. When a rotation of gp11 around its three-fold axis occurs, the end of the short tail fiber is oriented toward the host cell surface.

~~Interaction~~ of the short tail fibers with each other and with the gp11 ~~maintains~~ the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 and gp11 is essential for the attachment of gp12 to the baseplate. When a rotation of gp11 around its three-fold axis occurs, the end of the short tail fiber is oriented toward the host cell surface.

After the creation of six wedge composed of 3 gp11, ~~3gp10~~, 1 gp7, 2 gp8, 2 gp6, gp25 and gp53, is the wedge associated to the hub. Then are 3 gp9 and ~~3gp12~~ added.

After the creation of six wedge composed of gp11, gp10, gp7, gp8, gp6, gp25 and gp53, is the wedge associated to the hub. The hub is a complex of gp5-gp27. Then are gp9 and gp12 added. The chaperone protein gp57 help gp12 to adopt its correct folding into trimmers. To finish, gp48 and gp54 are added and it form the complete baseplate.

Sandbox Reserved 717: Difference between revisions

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@@ Line 32: / Line 32: @@
 To finish, the phage DNA single-stranded is injected into the bacterial cytoplasm through the tail tube.
-== '''Role of gp12''' ==
+== '''Presentation of gp12''' ==
-During the first step of the lytic cycle, the short tail fibers gp12 is attached to the host cell.
+During the first step of the lytic cycle, the short tail fibers gp12 attach the virion to the host cell. Thanks to a three-dimensional cryoelectron microscopy, a reconstruction of the baseplate was determined to a resolution of 12 Angstrom. It has shown that the total length of the gp12 density is inferior at 340 Å.
-A monomer of gp12 has a mass of 55.3 kDa. Gp12 can be divided into two fragments with different masses. Each short tail is composed of three repetitions of the monomer. Thanks to a three-dimensional cryoelectron microscopy, a reconstruction of the baseplate was determined to a resolution of 12 A.
+This technique was also a way to determine the interactions between the different proteins and their localization. Thus, interactions of the short tail fibers with each other and with the gp11 maintain the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 and gp11 is essential for the attachment of gp12 to the baseplate. When a rotation of gp11 around its three-fold axis occurs, the end of the short tail fiber is oriented toward the host cell surface.
-Interaction of the short tail fibers with each other and with the gp11 maintains the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 and gp11 is essential for the attachment of gp12 to the baseplate. When a rotation of gp11 around its three-fold axis occurs, the end of the short tail fiber is oriented toward the host cell surface.
-After the creation of six wedge composed of 3 gp11, 3gp10, 1 gp7, 2 gp8, 2 gp6, gp25 and gp53, is the wedge associated to the hub. Then are 3 gp9 and 3gp12 added.
+After the creation of six wedge composed of gp11, gp10, gp7, gp8, gp6, gp25 and gp53, is the wedge associated to the hub. The hub is a complex of gp5-gp27. Then are gp9 and gp12 added. The chaperone protein gp57 help gp12 to adopt its correct folding into trimmers. To finish, gp48 and gp54 are added and it form the complete baseplate.