Sandbox Reserved 717: Difference between revisions
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==='''The 45kDa Fragment <ref name=r2> doi:10.1038/nsb970</ref>''' === | ==='''The 45kDa Fragment <ref name=r2> doi:10.1038/nsb970</ref>''' === | ||
For generating the 45kDa fragment the full length gp12 was co-expressed with its chaperone gp57 and purified | For generating the 45kDa fragment the full length gp12 was co-expressed with its chaperone gp57 and purified <ref name=r1>doi:10.1186/1743-422X-7-355</ref>. Like the 33kDa fragment it also starts with the amino acid Leu85. The 45kDa fragment contains the residues <scene name='Sandbox_Reserved_717/Mythirdscene/4'>397-517</scene> , which are in the 33kDa fragment internal deleted. | ||
Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called head (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene> (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site. | Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called head (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene> (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site. | ||
