TAL effector: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
== | ==DNA binding module with marked RVD== | ||
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<br><br>[[Image:sanjana.png]]<br><br> | <br><br>[[Image:sanjana.png]]<br><br> | ||
==Reference== | ==Reference== | ||
Revision as of 15:59, 2 January 2013
Transcription activator-like effector PthXo1 (TALE PthXo1)TALEs are proteins originally derived from the plant pathogene Xanthomonas spp. The gene encoding for the effector are Pthxo1 and Pxo_00227. Originally they were detected in Xanthomonas Oryzae but the TALE PthXo1 genes were transformed into Escherichia Coli to express the DNA-binding protein.
3D structures of TALE PthXo1
2jg2 – PpSPT + aldimine – Pseudomonas paucimobilis
2jgt – PpSPT
2w8j - SpSPT + aldimine – Sphingomonas paucimobilis
2w8t, 2w8u, 2w8v, 2w8w - SpSPT (mutant) + aldimine
2xbn – SpSPT + pyridoxamine phosphate
3a2b – SPT + pyridoxal phosphate + serine – Sphingobacterium multivorum
About this Structure
DNA binding module with marked RVD
Reference
- Mak AN, Bradley P, Cernadas RA, Bogdanove AJ, Stoddard BL. The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target. Science. 2012 Jan 5. PMID:22223736 doi:10.1126/science.1216211
- Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F. A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. PMID:22222791 doi:10.1038/nprot.2011.431
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