Sandbox Reserved 717: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 31: | Line 31: | ||
== '''Presentation of gp12''' == | |||
<StructureSection load='1ocy' size='400' frame='true' align='right' caption='1ocy' scene='> | <StructureSection load='1ocy' size='400' frame='true' align='right' caption='1ocy' scene='> | ||
During the first step of the lytic cycle, the short tail fibers gp12 attach the virion to the host cell. | During the first step of the lytic cycle, the short tail fibers gp12 attach the virion to the host cell. | ||
| Line 38: | Line 39: | ||
This technique was also a way to determine the interactions between the different proteins and their localization. Thus, interactions of the short tail fibers with each other and with the gp11 maintain the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 | This technique was also a way to determine the interactions between the different proteins and their localization. Thus, interactions of the short tail fibers with each other and with the gp11 maintain the hexagon shape stability. The gp11 is also associated with gp10, which is clamped between the three fingers of gp11. This association between gp10 | ||
Short tail fibres consist of the single protein <scene name='Sandbox_Reserved_717/Myscene/1'>gp12</scene> . This protein forms a parallel, in-register, homo-trimer of 527 residues per subunit <ref name=r3>PMID: 12888344</ref>. 18 monomers are on the baseplate. 1ocy is a monomer of the short tail fibres. A monomer of gp12 has a mass of 55.3 kDa. The gp12 N-terminal domain is bound to the baseplate. While the C-terminal globular domain is supposed to bind the bacterial host cell <ref name=r3>PMID: 12888344</ref>. | Short tail fibres consist of the single protein <scene name='Sandbox_Reserved_717/Myscene/1'>gp12</scene> . This protein forms a parallel, in-register, homo-trimer of 527 residues per subunit <ref name=r3>PMID: 12888344</ref>. 18 monomers are on the baseplate. 1ocy is a monomer of the short tail fibres. A monomer of gp12 has a mass of 55.3 kDa. The gp12 N-terminal domain is bound to the baseplate. While the C-terminal globular domain is supposed to bind the bacterial host cell <ref name=r3>PMID: 12888344</ref>. | ||
| Line 71: | Line 71: | ||
'''It can not be ruled out that also other amino acids are important for binding ! <ref name=r3>PMID: 12888344</ref> Further it can not be ruled out that not all of these named amino acids are important for binding.''' | '''It can not be ruled out that also other amino acids are important for binding ! <ref name=r3>PMID: 12888344</ref> Further it can not be ruled out that not all of these named amino acids are important for binding.''' | ||
The mentioned amino acids are starting point for site-directed mutations and /or other biochemical experiments to clarify the location of this binding site. | The mentioned amino acids are starting point for site-directed mutations and /or other biochemical experiments to clarify the location of this binding site. | ||
</StructureSection> | |||
=='''Ligands and their Binding-Sites'''== | =='''Ligands and their Binding-Sites'''== | ||
| Line 88: | Line 89: | ||
*[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1ocy&template=ligands.html&o=METAL&l=1.1 Click here to see the interaction between the zinc ion and the two His amino acids] | *[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1ocy&template=ligands.html&o=METAL&l=1.1 Click here to see the interaction between the zinc ion and the two His amino acids] | ||
== '''Applications''' == | == '''Applications''' == | ||
