Sandbox Reserved 714: Difference between revisions
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=== N-terminal domain === | === N-terminal domain === | ||
The N-terminal domain is responsible of the Mg<sup>2+</sup> dependant hydrolysis of dihydroxy lipid phosphates <ref>PMID:15096040</ref>. Indeed, the aliphatic substrate binds the protein on its hydrophobic tunnel, as it has been described previously. The specificity of this enzyme has been tested for several lipid molecules, and the best substrate found is the monophosphate of dihydroxy stearic acid (threo-9/10-phosphonoxy-hydroxy-octadecanoic acid) <ref>PMID:12574510</ref>. Indeed, the catalytic values for this substrate are K<sub>m</sub> = 21 +/- 0.3 | The N-terminal domain is responsible of the Mg<sup>2+</sup> dependant hydrolysis of dihydroxy lipid phosphates <ref>PMID:15096040</ref>. Indeed, the aliphatic substrate binds the protein on its hydrophobic tunnel, as it has been described previously. The specificity of this enzyme has been tested for several lipid molecules, and the best substrate found is the monophosphate of dihydroxy stearic acid (threo-9/10-phosphonoxy-hydroxy-octadecanoic acid) <ref>PMID:12574510</ref>. Indeed, the catalytic values for this substrate are K<sub>m</sub> = 21 +/- 0.3 μM, V<sub>Max</sub> = 338 +/- 12 nmol.min<sup>-1</sup>.mg<sup>-1</sup>, and k<sub>cat</sub> = 0.35 +/- 0.01 s<sup>-1</sup>. | ||
In the example of this substrate, the reaction follows this equation: | In the example of this substrate, the reaction follows this equation: | ||
