1tgl: Difference between revisions
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New page: left|200px<br /><applet load="1tgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tgl, resolution 1.9Å" /> '''A SERINE PROTEASE TRI... |
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[[Image:1tgl.jpg|left|200px]]<br /><applet load="1tgl" size=" | [[Image:1tgl.jpg|left|200px]]<br /><applet load="1tgl" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1tgl, resolution 1.9Å" /> | caption="1tgl, resolution 1.9Å" /> | ||
'''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE'''<br /> | '''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE'''<br /> | ||
==Overview== | ==Overview== | ||
True lipases attach triacylglycerols and act at an oil-water interface; | True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop. | ||
==About this Structure== | ==About this Structure== | ||
1TGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http:// | 1TGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Triacylglycerol lipase]] | [[Category: Triacylglycerol lipase]] | ||
[[Category: Brady, L.]] | [[Category: Brady, L.]] | ||
[[Category: Brzozowski, A | [[Category: Brzozowski, A M.]] | ||
[[Category: Christiansen, L.]] | [[Category: Christiansen, L.]] | ||
[[Category: Derewenda, Z | [[Category: Derewenda, Z S.]] | ||
[[Category: Dodson, E | [[Category: Dodson, E J.]] | ||
[[Category: Dodson, G | [[Category: Dodson, G G.]] | ||
[[Category: Huge-Jensen, B.]] | [[Category: Huge-Jensen, B.]] | ||
[[Category: Norskov, L.]] | [[Category: Norskov, L.]] | ||
[[Category: Thim, L.]] | [[Category: Thim, L.]] | ||
[[Category: Tolley, S | [[Category: Tolley, S P.]] | ||
[[Category: Turkenburg, J | [[Category: Turkenburg, J P.]] | ||
[[Category: hydrolase(carboxylic esterase)]] | [[Category: hydrolase(carboxylic esterase)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:17 2008'' | ||
