1tmc: Difference between revisions

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==Overview==

Class I major histocompatibility complex (MHC) molecules are ternary, complexes of the soluble serum protein beta 2-microglobulin, MHC heavy, chain, and bound peptide. The first two domains (alpha 1, alpha 2) of the, heavy chain create the peptide binding cleft and the surface that contacts, the T-cell receptor. The third domain (alpha 3) associates with the T-cell, co-receptor, CD8, during T-cell recognition. Here we describe the x-ray, crystal structure of a human class I MHC molecule, HLA-Aw68, from which, the alpha 3 domain has been proteolytically removed. The resulting, molecule shows no gross morphological changes compared to the intact, protein. A decameric peptide complexed with the intact HLA-Aw68 is seen to, bind to the proteolized molecule in the conventional manner, demonstrating, that the alpha 3 domain is not required for the structural integrity of, the molecule or for peptide binding.

Class I major histocompatibility complex (MHC) molecules are ternary complexes of the soluble serum protein beta 2-microglobulin, MHC heavy chain, and bound peptide. The first two domains (alpha 1, alpha 2) of the heavy chain create the peptide binding cleft and the surface that contacts the T-cell receptor. The third domain (alpha 3) associates with the T-cell co-receptor, CD8, during T-cell recognition. Here we describe the x-ray crystal structure of a human class I MHC molecule, HLA-Aw68, from which the alpha 3 domain has been proteolytically removed. The resulting molecule shows no gross morphological changes compared to the intact protein. A decameric peptide complexed with the intact HLA-Aw68 is seen to bind to the proteolized molecule in the conventional manner, demonstrating that the alpha 3 domain is not required for the structural integrity of the molecule or for peptide binding.

==Disease==

Line 16:

[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Collins, E.J.]]

[[Category: Collins, E J.]]

[[Category: Garboczi, D.N.]]

[[Category: Garboczi, D N.]]

[[Category: Karpusas, M.N.]]

[[Category: Karpusas, M N.]]

[[Category: Wiley, D.C.]]

[[Category: Wiley, D C.]]

[[Category: histocompatibility antigen]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:15:07 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Class I major histocompatibility complex (MHC) molecules are ternary, complexes of the soluble serum protein beta 2-microglobulin, MHC heavy, chain, and bound peptide. The first two domains (alpha 1, alpha 2) of the, heavy chain create the peptide binding cleft and the surface that contacts, the T-cell receptor. The third domain (alpha 3) associates with the T-cell, co-receptor, CD8, during T-cell recognition. Here we describe the x-ray, crystal structure of a human class I MHC molecule, HLA-Aw68, from which, the alpha 3 domain has been proteolytically removed. The resulting, molecule shows no gross morphological changes compared to the intact, protein. A decameric peptide complexed with the intact HLA-Aw68 is seen to, bind to the proteolized molecule in the conventional manner, demonstrating, that the alpha 3 domain is not required for the structural integrity of, the molecule or for peptide binding.
+Class I major histocompatibility complex (MHC) molecules are ternary complexes of the soluble serum protein beta 2-microglobulin, MHC heavy chain, and bound peptide. The first two domains (alpha 1, alpha 2) of the heavy chain create the peptide binding cleft and the surface that contacts the T-cell receptor. The third domain (alpha 3) associates with the T-cell co-receptor, CD8, during T-cell recognition. Here we describe the x-ray crystal structure of a human class I MHC molecule, HLA-Aw68, from which the alpha 3 domain has been proteolytically removed. The resulting molecule shows no gross morphological changes compared to the intact protein. A decameric peptide complexed with the intact HLA-Aw68 is seen to bind to the proteolized molecule in the conventional manner, demonstrating that the alpha 3 domain is not required for the structural integrity of the molecule or for peptide binding.
 ==Disease==
@@ Line 16: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Collins, E.J.]]
+[[Category: Collins, E J.]]
-[[Category: Garboczi, D.N.]]
+[[Category: Garboczi, D N.]]
-[[Category: Karpusas, M.N.]]
+[[Category: Karpusas, M N.]]
-[[Category: Wiley, D.C.]]
+[[Category: Wiley, D C.]]
 [[Category: histocompatibility antigen]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:57:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:15:07 2008''