Sandbox Reserved 709: Difference between revisions
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* '''Step 6 :''' '''Nup50''' (also called '''Npap60'''), a nucleoplasmin, weakens the link between importin α and the NLS of the cargo. Thus the cargo protein is now free in the nucleoplasm. | * '''Step 6 :''' '''Nup50''' (also called '''Npap60'''), a nucleoplasmin, weakens the link between importin α and the NLS of the cargo. Thus the cargo protein is now free in the nucleoplasm. | ||
* '''Step 7 :''' Importin α can’t leave the nucleus only with the help of Ran-GTP. It needs also interaction with '''CAS''' (''Cellular Apoptosis Susceptibility'' protein), an '''exportin''' which is similar in shape to importin β. Binding between CAS and importin α is favoured by interactions with Nup50. | * '''Step 7 :''' Importin α can’t leave the nucleus only with the help of Ran-GTP. It needs also interaction with '''CAS''' (''Cellular Apoptosis Susceptibility'' protein), an '''exportin''' which is similar in shape to importin β. Binding between CAS and importin α is favoured by interactions with Nup50. | ||
* '''Step 8 :'''The newly formed '''importin α:CAS:Ran-GTP''' complex is able to interact with nucleoplasmins of NPC, and once in the cytoplasm, Ran-GTP is hydrolysed into Ran-GDP (''see '''Step 5''' for details''). The complex splits, and importin α is now ready for a new import cycle. | * '''Step 8 :'''The newly formed '''importin α:CAS:Ran-GTP''' complex is able to interact with nucleoplasmins of NPC, and once in the cytoplasm, Ran-GTP is hydrolysed into Ran-GDP (''see '''Step 5''' for details''). The complex splits, and importin α is now ready for a new import cycle.<ref>PMID:016222336<ref/> | ||
==Importin α structure== | ==Importin α structure== | ||
