Sandbox Reserved 704: Difference between revisions
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== Identity elements involved in complex formation == | == Identity elements involved in complex formation == | ||
<StructureSection load='1wz2' size=' | <StructureSection load='1wz2' size='400' frame='true' align='left' side='right' caption='The crystal structure of Leucyl-tRNA synthetase and tRNA(leucine) complex(PDB entry [[1wz2]])' scene=''/> | ||
Most aaRSs interact with the tRNA anticodon loop in order to form aminoacyl-tRNA <scene name='Sandbox_Reserved_704/Trna_enzyme_complex/1'>complex</scene>. But in the case of archaeal LeuRSs, none of them use this recognition mode. In fact, LeuRSs use two identity elements: the discriminator A73 and the long variable arm <ref name="Fukunaga">PMID:15663927</ref>. | Most aaRSs interact with the tRNA anticodon loop in order to form aminoacyl-tRNA <scene name='Sandbox_Reserved_704/Trna_enzyme_complex/1'>complex</scene>. But in the case of archaeal LeuRSs, none of them use this recognition mode. In fact, LeuRSs use two identity elements: the discriminator A73 and the long variable arm <ref name="Fukunaga">PMID:15663927</ref>. | ||
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The C-terminal region of LeuRS is composed of β-sheet surrounded by α-helices and an additional region (a structure with α- α - α topology), specific to this enzyme. At the tip of the long variable arm of tRNA Leu, there are four nucleotides: G47a, U47b, A47c and G47d which formed a loop. Some of these nucleotides interact with C-terminal residues through Van der Waals interactions and hydrogen bonds. | The C-terminal region of LeuRS is composed of β-sheet surrounded by α-helices and an additional region (a structure with α- α - α topology), specific to this enzyme. At the tip of the long variable arm of tRNA Leu, there are four nucleotides: G47a, U47b, A47c and G47d which formed a loop. Some of these nucleotides interact with C-terminal residues through Van der Waals interactions and hydrogen bonds. | ||
First, A47c and G47d which are turned outward of the loop interact with <scene name='Sandbox_Reserved_704/Pro962/2'>Pro962</scene> and <scene name='Sandbox_Reserved_704/Glu_967/1'>Glu967</scene>. | First, A47c and G47d which are turned outward of the loop interact with <scene name='Sandbox_Reserved_704/Pro962/2'>Pro962</scene> and <scene name='Sandbox_Reserved_704/Glu_967/1'>Glu967</scene>. | ||
Then, A47c interacts specifically with <scene name='Sandbox_Reserved_704/Ile849_ile966_glu967/1'> | Then, A47c interacts specifically with <scene name='Sandbox_Reserved_704/Ile849_ile966_glu967/1'>3 residues</scene>: Ile849, Ile966 and Glu967. And G47d interacts with <scene name='Sandbox_Reserved_704/4_residues/1'>4 residues</scene>: Ile 849, Ile964, Asp845 and Pro962. | ||
Both A47c and G47d recognition by C-terminal region of the enzyme are important for recognition of the variable arm and a correct translation process <ref name="">PMID:16155584</ref>. | Both A47c and G47d recognition by C-terminal region of the enzyme are important for recognition of the variable arm and a correct translation process <ref name="">PMID:16155584</ref>. | ||
