1tpz: Difference between revisions
New page: left|200px<br /><applet load="1tpz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tpz, resolution 2.0Å" /> '''Crystal Structure of ... |
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[[Image:1tpz.jpg|left|200px]]<br /><applet load="1tpz" size=" | [[Image:1tpz.jpg|left|200px]]<br /><applet load="1tpz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1tpz, resolution 2.0Å" /> | caption="1tpz, resolution 2.0Å" /> | ||
'''Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases'''<br /> | '''Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases'''<br /> | ||
==Overview== | ==Overview== | ||
Interferon-inducible p47 GTPases are critical mediators of cell-autonomous | Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases. | ||
==About this Structure== | ==About this Structure== | ||
1TPZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, GDP, EDO and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1TPZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GDP:'>GDP</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: interferon gamma]] | [[Category: interferon gamma]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:16:12 2008'' | ||
Revision as of 13:16, 21 February 2008
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Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases
Overview
Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.
About this Structure
1TPZ is a Single protein structure of sequence from Mus musculus with MG, GDP, EDO and MPD as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases., Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E, Mol Cell. 2004 Sep 10;15(5):727-39. PMID:15350217
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