Sandbox Reserved 706: Difference between revisions
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To obtain structural information on the complex of mouse Rab27B and Slac-a, a C-terminally truncated form of the GTPase-deficient mutant Rab27B (Q78L) was used. | To obtain structural information on the complex of mouse Rab27B and Slac-a, a C-terminally truncated form of the GTPase-deficient mutant Rab27B (Q78L) was used. | ||
In fact, full size of Rab27B is 218 amino acids long whereas the truncated form of Rab27B contains only the GTPase domain (residues 1 to 201). <ref>PMID:18940604</ref> <ref>http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zet</ref> | In fact, full size of Rab27B is 218 amino acids long whereas the truncated form of Rab27B contains only the GTPase domain (residues 1 to 201). <ref>PMID:18940604</ref> <ref>http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zet</ref> | ||
[[Image:Image:Rab27B slac2a image1.jpg]] | |||
'''Detailed structure:''' | '''Detailed structure:''' | ||
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Rab27B–GTP adopts a compact monomer conformation, while an extended conformation was observed for GDP-bound Rab27B. | Rab27B–GTP adopts a compact monomer conformation, while an extended conformation was observed for GDP-bound Rab27B. | ||
Switch 1 and switch 2 of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with its γ-phosphate, and this way also allow Slac2-a binding.(see next paragraph) But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so that there are no inter-actions with Slac2-a and no binding. | Switch 1 and switch 2 of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with its γ-phosphate, and this way also allow Slac2-a binding.(see next paragraph) But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so that there are no inter-actions with Slac2-a and no binding. | ||
=== '''Strucure Slac2-a''' === | === '''Strucure Slac2-a''' === | ||
