1txz: Difference between revisions
New page: left|200px<br /><applet load="1txz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1txz, resolution 2.05Å" /> '''Crystal structure of... |
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[[Image:1txz.jpg|left|200px]]<br /><applet load="1txz" size=" | [[Image:1txz.jpg|left|200px]]<br /><applet load="1txz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1txz, resolution 2.05Å" /> | caption="1txz, resolution 2.05Å" /> | ||
'''Crystal structure of yeast ymx7, an ADP-ribose-1''-monophosphatase, complexed with ADP-ribose'''<br /> | '''Crystal structure of yeast ymx7, an ADP-ribose-1''-monophosphatase, complexed with ADP-ribose'''<br /> | ||
==Overview== | ==Overview== | ||
Appr-1''-pase, an important and ubiquitous cellular processing enzyme | Appr-1''-pase, an important and ubiquitous cellular processing enzyme involved in the tRNA splicing pathway, catalyzes the conversion of ADP-ribose-1''monophosphate (Appr-1''-p) to ADP-ribose. The structures of the native enzyme from the yeast and its complex with ADP-ribose were determined to 1.9 A and 2.05 A, respectively. Analysis of the three-dimensional structure of this protein, selected as a target in a structural genomics project, reveals its putative function and provides clues to the catalytic mechanism. The structure of the 284-amino acid protein shows a two-domain architecture consisting of a three-layer alphabetaalpha sandwich N-terminal domain connected to a small C-terminal helical domain. The structure of Appr-1''-pase in complex with the product, ADP-ribose, reveals an active-site water molecule poised for nucleophilic attack on the terminal phosphate group. Loop-region residues Asn 80, Asp 90, and His 145 may form a catalytic triad. | ||
==About this Structure== | ==About this Structure== | ||
1TXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4, NA, APR and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1TXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=APR:'>APR</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Kumaran, D.]] | [[Category: Kumaran, D.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: APR]] | [[Category: APR]] | ||
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[[Category: two domain organization]] | [[Category: two domain organization]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:31 2008'' | ||
Revision as of 13:18, 21 February 2008
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Crystal structure of yeast ymx7, an ADP-ribose-1-monophosphatase, complexed with ADP-ribose
Overview
Appr-1-pase, an important and ubiquitous cellular processing enzyme involved in the tRNA splicing pathway, catalyzes the conversion of ADP-ribose-1monophosphate (Appr-1-p) to ADP-ribose. The structures of the native enzyme from the yeast and its complex with ADP-ribose were determined to 1.9 A and 2.05 A, respectively. Analysis of the three-dimensional structure of this protein, selected as a target in a structural genomics project, reveals its putative function and provides clues to the catalytic mechanism. The structure of the 284-amino acid protein shows a two-domain architecture consisting of a three-layer alphabetaalpha sandwich N-terminal domain connected to a small C-terminal helical domain. The structure of Appr-1-pase in complex with the product, ADP-ribose, reveals an active-site water molecule poised for nucleophilic attack on the terminal phosphate group. Loop-region residues Asn 80, Asp 90, and His 145 may form a catalytic triad.
About this Structure
1TXZ is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4, NA, APR and EDO as ligands. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of ADP-ribose-1-monophosphatase (Appr-1-pase), a ubiquitous cellular processing enzyme., Kumaran D, Eswaramoorthy S, Studier FW, Swaminathan S, Protein Sci. 2005 Mar;14(3):719-26. PMID:15722447
Page seeded by OCA on Thu Feb 21 15:18:31 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Saccharomyces cerevisiae
- Single protein
- Burley, S K.
- Kumaran, D.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Swaminathan, S.
- APR
- EDO
- NA
- SO4
- Adp-ribose complex
- Dimer
- New york structural genomix research consortium
- Nysgxrc
- Protein structure initiative
- Psi
- Structural genomics
- Two domain organization
