1txu: Difference between revisions

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==Overview==

The Rab5 GTPase, an essential regulator of endocytosis and endosome, biogenesis, is activated by guanine-nucleotide exchange factors (GEFs), that contain a Vps9 domain. Here, we show that the catalytic core of the, Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain, stabilized by an indispensable helical bundle. A family-wide analysis of, Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases., Conserved exchange determinants map to a common surface of the Vps9, domain, which recognizes invariant aromatic residues in the switch regions, of Rab GTPases and selects for the Rab5 subfamily by requiring a small, nonacidic residue preceding a critical phenylalanine in the switch I, region. These and other observations reveal unexpected similarity with the, Arf exchange site in the Sec7 domain.

The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.

==About this Structure==

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[[Category: Single protein]]

[[Category: Delprato, A.]]

[[Category: Lambright, D.G.]]

[[Category: Lambright, D G.]]

[[Category: Merithew, E.]]

[[Category: MG]]

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[[Category: vps9 domain]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:30 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The Rab5 GTPase, an essential regulator of endocytosis and endosome, biogenesis, is activated by guanine-nucleotide exchange factors (GEFs), that contain a Vps9 domain. Here, we show that the catalytic core of the, Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain, stabilized by an indispensable helical bundle. A family-wide analysis of, Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases., Conserved exchange determinants map to a common surface of the Vps9, domain, which recognizes invariant aromatic residues in the switch regions, of Rab GTPases and selects for the Rab5 subfamily by requiring a small, nonacidic residue preceding a critical phenylalanine in the switch I, region. These and other observations reveal unexpected similarity with the, Arf exchange site in the Sec7 domain.
+The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Delprato, A.]]
-[[Category: Lambright, D.G.]]
+[[Category: Lambright, D G.]]
 [[Category: Merithew, E.]]
 [[Category: MG]]
@@ Line 21: / Line 21: @@
 [[Category: vps9 domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:58:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:30 2008''