1u02: Difference between revisions
New page: left|200px<br /><applet load="1u02" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u02, resolution 1.92Å" /> '''Crystal structure of... |
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[[Image:1u02.gif|left|200px]]<br /><applet load="1u02" size=" | [[Image:1u02.gif|left|200px]]<br /><applet load="1u02" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1u02, resolution 1.92Å" /> | caption="1u02, resolution 1.92Å" /> | ||
'''Crystal structure of trehalose-6-phosphate phosphatase related protein'''<br /> | '''Crystal structure of trehalose-6-phosphate phosphatase related protein'''<br /> | ||
==Overview== | ==Overview== | ||
We report here the crystal structure of a trehalose-6-phosphate | We report here the crystal structure of a trehalose-6-phosphate phosphatase-related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual-wavelength anomalous diffraction (DAD) method. T6PP is a member of the haloacid dehalogenase (HAD) superfamily with significant sequence homology with trehalose-6-phosphate phosphatase, phosphoserine phosphatase, P-type ATPases and other members of the family. T6PP possesses a core domain of known alpha/beta-hydrolase fold, characteristic of the HAD family, and a cap domain, with a tertiary fold consisting of a four-stranded beta-sheet with two alpha-helices on one side of the sheet. An active-site magnesium ion and a glycerol molecule bound at the interface between the two domains provide insight into the mode of substrate binding by T6PP. A trehalose-6-phosphate molecule modeled into a cage formed by the two domains makes favorable interactions with the protein molecule. We have confirmed that T6PP is a trehalose phosphatase from amino acid sequence, three-dimensional structure, and biochemical assays. | ||
==About this Structure== | ==About this Structure== | ||
1U02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] with MG, NA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1U02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U02 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermoplasma acidophilum]] | [[Category: Thermoplasma acidophilum]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Krishnamurthy, N | [[Category: Krishnamurthy, N R.]] | ||
[[Category: Kumaran, D.]] | [[Category: Kumaran, D.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:13 2008'' | ||
Revision as of 13:19, 21 February 2008
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Crystal structure of trehalose-6-phosphate phosphatase related protein
Overview
We report here the crystal structure of a trehalose-6-phosphate phosphatase-related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual-wavelength anomalous diffraction (DAD) method. T6PP is a member of the haloacid dehalogenase (HAD) superfamily with significant sequence homology with trehalose-6-phosphate phosphatase, phosphoserine phosphatase, P-type ATPases and other members of the family. T6PP possesses a core domain of known alpha/beta-hydrolase fold, characteristic of the HAD family, and a cap domain, with a tertiary fold consisting of a four-stranded beta-sheet with two alpha-helices on one side of the sheet. An active-site magnesium ion and a glycerol molecule bound at the interface between the two domains provide insight into the mode of substrate binding by T6PP. A trehalose-6-phosphate molecule modeled into a cage formed by the two domains makes favorable interactions with the protein molecule. We have confirmed that T6PP is a trehalose phosphatase from amino acid sequence, three-dimensional structure, and biochemical assays.
About this Structure
1U02 is a Single protein structure of sequence from Thermoplasma acidophilum with MG, NA and GOL as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications., Rao KN, Kumaran D, Seetharaman J, Bonanno JB, Burley SK, Swaminathan S, Protein Sci. 2006 Jul;15(7):1735-44. PMID:16815921
Page seeded by OCA on Thu Feb 21 15:19:13 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Single protein
- Thermoplasma acidophilum
- Burley, S K.
- Krishnamurthy, N R.
- Kumaran, D.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Swaminathan, S.
- GOL
- MG
- NA
- New york structural genomix research consortium
- Nysgxrc
- Phosphatase
- Protein structure initiative
- Psi
- Structural genomics
