1u7f: Difference between revisions

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==Overview==

The formation of protein complexes between phosphorylated R-Smads and, Smad4 is a central event in the TGF-beta signaling pathway. We have, determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both, complexes are heterotrimers, comprising two phosphorylated R-Smad subunits, and one Smad4 subunit, a finding that was corroborated by isothermal, titration calorimetry and mutational studies. Preferential formation of, the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely, enthalpy driven, contributed by the unique presence of strong, electrostatic interactions within the heterotrimeric interfaces. The study, supports a common mechanism of Smad protein assembly in TGF-beta, superfamily signaling.

The formation of protein complexes between phosphorylated R-Smads and Smad4 is a central event in the TGF-beta signaling pathway. We have determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both complexes are heterotrimers, comprising two phosphorylated R-Smad subunits and one Smad4 subunit, a finding that was corroborated by isothermal titration calorimetry and mutational studies. Preferential formation of the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely enthalpy driven, contributed by the unique presence of strong electrostatic interactions within the heterotrimeric interfaces. The study supports a common mechanism of Smad protein assembly in TGF-beta superfamily signaling.

==Disease==

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[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Caestecker, M.de.]]

[[Category: Caestecker, M de.]]

[[Category: Chacko, B.M.]]

[[Category: Chacko, B M.]]

[[Category: Hayward, L.J.]]

[[Category: Hayward, L J.]]

[[Category: Lam, S.]]

[[Category: Lin, K.]]

[[Category: Qin, B.Y.]]

[[Category: Qin, B Y.]]

[[Category: Shi, G.]]

[[Category: Tiwari, A.]]

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[[Category: tgf-beta]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:21 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The formation of protein complexes between phosphorylated R-Smads and, Smad4 is a central event in the TGF-beta signaling pathway. We have, determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both, complexes are heterotrimers, comprising two phosphorylated R-Smad subunits, and one Smad4 subunit, a finding that was corroborated by isothermal, titration calorimetry and mutational studies. Preferential formation of, the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely, enthalpy driven, contributed by the unique presence of strong, electrostatic interactions within the heterotrimeric interfaces. The study, supports a common mechanism of Smad protein assembly in TGF-beta, superfamily signaling.
+The formation of protein complexes between phosphorylated R-Smads and Smad4 is a central event in the TGF-beta signaling pathway. We have determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both complexes are heterotrimers, comprising two phosphorylated R-Smad subunits and one Smad4 subunit, a finding that was corroborated by isothermal titration calorimetry and mutational studies. Preferential formation of the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely enthalpy driven, contributed by the unique presence of strong electrostatic interactions within the heterotrimeric interfaces. The study supports a common mechanism of Smad protein assembly in TGF-beta superfamily signaling.
 ==Disease==
@@ Line 16: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Caestecker, M.de.]]
+[[Category: Caestecker, M de.]]
-[[Category: Chacko, B.M.]]
+[[Category: Chacko, B M.]]
-[[Category: Hayward, L.J.]]
+[[Category: Hayward, L J.]]
 [[Category: Lam, S.]]
 [[Category: Lin, K.]]
-[[Category: Qin, B.Y.]]
+[[Category: Qin, B Y.]]
 [[Category: Shi, G.]]
 [[Category: Tiwari, A.]]
@@ Line 30: / Line 30: @@
 [[Category: tgf-beta]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:59:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:21 2008''