1u7k: Difference between revisions

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[[Image:1u7k.jpg|left|200px]]<br /><applet load="1u7k" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1u7k.jpg|left|200px]]<br /><applet load="1u7k" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1u7k, resolution 1.85Å" />

'''Structure of a hexameric N-terminal domain from murine leukemia virus capsid'''<br />

==Overview==

Retroviruses are the aetiological agents of a range of human diseases, including AIDS and T-cell leukaemias. They follow complex life cycles, which are still only partly understood at the molecular level. Maturation, of newly formed retroviral particles is an essential step in production of, infectious virions, and requires proteolytic cleavage of Gag polyproteins, in the immature particle to form the matrix, capsid and nucleocapsid, proteins present in the mature virion. Capsid proteins associate to form a, dense viral core that may be spherical, cylindrical or conical depending, on the genus of the virus. Nonetheless, these assemblies all appear to be, composed of a lattice formed from hexagonal rings, each containing six, capsid monomers. Here, we describe the X-ray structure of an individual, hexagonal assembly from N-tropic murine leukaemia virus (N-MLV). The, interface between capsid monomers is generally polar, consistent with weak, interactions within the hexamer. Similar architectures are probably, crucial for the regulation of capsid assembly and disassembly in all, retroviruses. Together, these observations provide new insights into, retroviral uncoating and how cellular restriction factors may interfere, with viral replication.

Retroviruses are the aetiological agents of a range of human diseases including AIDS and T-cell leukaemias. They follow complex life cycles, which are still only partly understood at the molecular level. Maturation of newly formed retroviral particles is an essential step in production of infectious virions, and requires proteolytic cleavage of Gag polyproteins in the immature particle to form the matrix, capsid and nucleocapsid proteins present in the mature virion. Capsid proteins associate to form a dense viral core that may be spherical, cylindrical or conical depending on the genus of the virus. Nonetheless, these assemblies all appear to be composed of a lattice formed from hexagonal rings, each containing six capsid monomers. Here, we describe the X-ray structure of an individual hexagonal assembly from N-tropic murine leukaemia virus (N-MLV). The interface between capsid monomers is generally polar, consistent with weak interactions within the hexamer. Similar architectures are probably crucial for the regulation of capsid assembly and disassembly in all retroviruses. Together, these observations provide new insights into retroviral uncoating and how cellular restriction factors may interfere with viral replication.

==About this Structure==

1U7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1U7K OCA].

1U7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7K OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Viruses]]

[[Category: Haire, L.F.]]

[[Category: Haire, L F.]]

[[Category: Mortuza, G.B.]]

[[Category: Mortuza, G B.]]

[[Category: Smerdon, S.J.]]

[[Category: Smerdon, S J.]]

[[Category: Stevens, A.]]

[[Category: Stoye, J.P.]]

[[Category: Stoye, J P.]]

[[Category: Taylor, I.A.]]

[[Category: Taylor, I A.]]

[[Category: capsid]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 03:54:~~01 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:22 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1u7k.jpg|left|200px]]<br /><applet load="1u7k" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1u7k.jpg|left|200px]]<br /><applet load="1u7k" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1u7k, resolution 1.85&Aring;" />
 '''Structure of a hexameric N-terminal domain from murine leukemia virus capsid'''<br />
 ==Overview==
-Retroviruses are the aetiological agents of a range of human diseases, including AIDS and T-cell leukaemias. They follow complex life cycles, which are still only partly understood at the molecular level. Maturation, of newly formed retroviral particles is an essential step in production of, infectious virions, and requires proteolytic cleavage of Gag polyproteins, in the immature particle to form the matrix, capsid and nucleocapsid, proteins present in the mature virion. Capsid proteins associate to form a, dense viral core that may be spherical, cylindrical or conical depending, on the genus of the virus. Nonetheless, these assemblies all appear to be, composed of a lattice formed from hexagonal rings, each containing six, capsid monomers. Here, we describe the X-ray structure of an individual, hexagonal assembly from N-tropic murine leukaemia virus (N-MLV). The, interface between capsid monomers is generally polar, consistent with weak, interactions within the hexamer. Similar architectures are probably, crucial for the regulation of capsid assembly and disassembly in all, retroviruses. Together, these observations provide new insights into, retroviral uncoating and how cellular restriction factors may interfere, with viral replication.
+Retroviruses are the aetiological agents of a range of human diseases including AIDS and T-cell leukaemias. They follow complex life cycles, which are still only partly understood at the molecular level. Maturation of newly formed retroviral particles is an essential step in production of infectious virions, and requires proteolytic cleavage of Gag polyproteins in the immature particle to form the matrix, capsid and nucleocapsid proteins present in the mature virion. Capsid proteins associate to form a dense viral core that may be spherical, cylindrical or conical depending on the genus of the virus. Nonetheless, these assemblies all appear to be composed of a lattice formed from hexagonal rings, each containing six capsid monomers. Here, we describe the X-ray structure of an individual hexagonal assembly from N-tropic murine leukaemia virus (N-MLV). The interface between capsid monomers is generally polar, consistent with weak interactions within the hexamer. Similar architectures are probably crucial for the regulation of capsid assembly and disassembly in all retroviruses. Together, these observations provide new insights into retroviral uncoating and how cellular restriction factors may interfere with viral replication.
 ==About this Structure==
-U7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U7K OCA].
+U7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7K OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Viruses]]
-[[Category: Haire, L.F.]]
+[[Category: Haire, L F.]]
-[[Category: Mortuza, G.B.]]
+[[Category: Mortuza, G B.]]
-[[Category: Smerdon, S.J.]]
+[[Category: Smerdon, S J.]]
 [[Category: Stevens, A.]]
-[[Category: Stoye, J.P.]]
+[[Category: Stoye, J P.]]
-[[Category: Taylor, I.A.]]
+[[Category: Taylor, I A.]]
 [[Category: capsid]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:54:01 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:22 2008''