1ufp: Difference between revisions

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[[Image:1ufp.jpg|left|200px]]<br /><applet load="1ufp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ufp.jpg|left|200px]]<br /><applet load="1ufp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ufp, resolution 2.10Å" />

'''Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-wild type Myoglobin'''<br />

==Overview==

Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with, FeIII(salophen) (1) (salophen =, N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen), (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of, 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric, repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests, a possible accommodation of a small substrate in the cavity. In fact, the, cyanide association rate constant of 2.apo-A71GMb is 216-fold larger, compared to that of 2.apo-Mb. These results provide us principles for the, noncovalent fixation of synthetic metal cofactors at the desired positions, in protein matrixes.

Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.

==About this Structure==

1UFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1UFP OCA].

1UFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFP OCA].

==Reference==

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[[Category: schiff base]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 04:05:~~35 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:24:00 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ufp.jpg|left|200px]]<br /><applet load="1ufp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ufp.jpg|left|200px]]<br /><applet load="1ufp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ufp, resolution 2.10&Aring;" />
 '''Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-wild type Myoglobin'''<br />
 ==Overview==
-Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with, FeIII(salophen) (1) (salophen =, N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen), (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of, 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric, repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests, a possible accommodation of a small substrate in the cavity. In fact, the, cyanide association rate constant of 2.apo-A71GMb is 216-fold larger, compared to that of 2.apo-Mb. These results provide us principles for the, noncovalent fixation of synthetic metal cofactors at the desired positions, in protein matrixes.
+Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.
 ==About this Structure==
-UFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UFP OCA].
+UFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFP OCA].
 ==Reference==
@@ Line 27: / Line 27: @@
 [[Category: schiff base]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:05:35 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:24:00 2008''