1ugl: Difference between revisions
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'''Solution structure of S8-SP11'''<br /> | '''Solution structure of S8-SP11'''<br /> | ||
==Overview== | ==Overview== | ||
Many flowering plants possess a self-incompatibility system to prevent | Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. | ||
==About this Structure== | ==About this Structure== | ||
1UGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1UGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Isogai, A.]] | [[Category: Isogai, A.]] | ||
[[Category: Jee, J | [[Category: Jee, J G.]] | ||
[[Category: Kojima, C.]] | [[Category: Kojima, C.]] | ||
[[Category: Mishima, M.]] | [[Category: Mishima, M.]] | ||
[[Category: RSGI, RIKEN | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | ||
[[Category: Sasaki, K.]] | [[Category: Sasaki, K.]] | ||
[[Category: Shirakawa, M.]] | [[Category: Shirakawa, M.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:24:15 2008'' | ||
Revision as of 13:24, 21 February 2008
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Solution structure of S8-SP11
Overview
Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor.
About this Structure
1UGL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the male determinant factor for Brassica self-incompatibility., Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M, J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:12835321
Page seeded by OCA on Thu Feb 21 15:24:15 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Single protein
- Isogai, A.
- Jee, J G.
- Kojima, C.
- Mishima, M.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Sasaki, K.
- Shirakawa, M.
- Takayama, S.
- Cysteine-rich
- Defensin-like
- Male determinant of self-incompatibility
- Nmr
- Riken structural genomics/proteomics initiative
- Rsgi
- Scr
- Sp11
- Structural genomics
