Sandbox Reserved 707: Difference between revisions
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It is very important to distinguish conserved amino acid residue signatures that constitute the catalytic core of the B-RAF Kinase. The catalytic properties are done by the KDD (Lys578-Asp576-Asp594) Motif and the activation segment starts in general with a DFG and ends with APE. But how the catalytic reaction is generated? | It is very important to distinguish conserved amino acid residue signatures that constitute the catalytic core of the B-RAF Kinase. The catalytic properties are done by the <scene name='Sandbox_Reserved_707/Kdd_motif/1'>KDD (Lys578-Asp576-Asp594)</scene> Motif and the activation segment starts in general with a DFG and ends with APE. But how the catalytic reaction is generated? | ||
An invariant lysine (Lys578 in B-RAF) forms salt bridges with the gamma phosphate of the ATP. Asp576, which is a base in the catalytic loop, orients the seryl or threonyl group of the substrate protein and takes the proton of the hydroxyl group, facilitating the attack of oxygen on the gamma phosphorus atom of MgATP. Asp594 binds Mg²⁺ which coordinates the beta and gamma phosphates of ATP. <br/> | An invariant lysine (<scene name='Sandbox_Reserved_707/K578/1'>Lys578</scene> in B-RAF) forms salt bridges with the gamma phosphate of the ATP. Asp576, which is a base in the catalytic loop, orients the seryl or threonyl group of the substrate protein and takes the proton of the hydroxyl group, facilitating the attack of oxygen on the gamma phosphorus atom of MgATP. Asp594 binds Mg²⁺ which coordinates the beta and gamma phosphates of ATP. <br/> | ||
[[Image:138 page3 image1.jpg|500px|thumb| Diagram of the inferred interactions between human B-RAF kinase catalytic core residues, ATP, and MEK]] | [[Image:138 page3 image1.jpg|500px|thumb| Diagram of the inferred interactions between human B-RAF kinase catalytic core residues, ATP, and MEK]] | ||
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|'''<scene name='Sandbox_Reserved_707/Hrd/2'>HRD</scene>''': || 574-576 | |'''<scene name='Sandbox_Reserved_707/Hrd/2'>HRD</scene>''': || 574-576 | ||
|- | |- | ||
|'''K of KDD''': || 578 | |'''<scene name='Sandbox_Reserved_707/K578/1'>K</scene> of <scene name='Sandbox_Reserved_707/Kdd_motif/1'>KDD</scene>''': || 578 | ||
|- | |- | ||
|'''DFG''': || 594-596 | |'''DFG''': || 594-596 | ||
