Sandbox Reserved 707: Difference between revisions
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|'''<scene name='Sandbox_Reserved_707/Dfg_start_activation_segment/1'>DFG</scene>''': || 594-596 | |'''<scene name='Sandbox_Reserved_707/Dfg_start_activation_segment/1'>DFG</scene>''': || 594-596 | ||
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|'''Activation segment phosphorylation sites''': || T599-S602 | |'''Activation segment phosphorylation sites''': || <scene name='Sandbox_Reserved_707/Psite_of_activation_segment/1'>T599</scene>-S602 | ||
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|'''End of Activation segment''': || APE 621-623 | |'''End of Activation segment''': || APE 621-623 | ||
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Under non stimulatory conditions a serine of CR2 domain and another near the C-terminus are phosphorylated and are bound to 14-3-3 domain. To activate RAF Kinases, RAS-GTP has to interact with the RDB Domain. This is necessary but not enough to activate the RAF Kinase. | Under non stimulatory conditions a serine of CR2 domain and another near the C-terminus are phosphorylated and are bound to 14-3-3 domain. To activate RAF Kinases, RAS-GTP has to interact with the RDB Domain. This is necessary but not enough to activate the RAF Kinase. | ||
One characteristic that distinguish B-RAF from A or C-RAF is the presence of Asp448 and Asp449 on the N-terminal region, which bear negative charges. Phosphorylation of Thr599 and Ser602 in the activation segment is essential for B-RAF activation. Another residue is crucial for the activation of this enzyme as well: the phosphoserine 579 of the catalytic loop. That’s why the basal activity of B-RAF is greater than A or C-RAF.<br /> | One characteristic that distinguish B-RAF from A or C-RAF is the presence of Asp448 and Asp449 on the N-terminal region, which bear negative charges. Phosphorylation of <scene name='Sandbox_Reserved_707/Psite_of_activation_segment/1'>Thr599</scene> and Ser602 in the activation segment is essential for B-RAF activation. Another residue is crucial for the activation of this enzyme as well: the phosphoserine 579 of the catalytic loop. That’s why the basal activity of B-RAF is greater than A or C-RAF.<br /> | ||
