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==Overview==

A combined NMR-molecular dynamics approach has been applied to determine, the solution structure of a HIV-1 17-mer rev peptide bound to its 35-mer, high affinity RNA aptamer binding site. Complex formation involves, adaptive binding with the alpha-helical arginine-rich basic rev peptide, targeting a widened RNA major groove centred about adjacent G.A and, reversed A.A mismatches. We have also identified a U AU triple in the, aptamer complex with the Hoogsteen-paired uracil base sandwiched between, two arginine side chains. The intermolecular contacts identified in the, aptamer complex readily account for the consequences of peptide and RNA, mutations, as well as the results of previous in vitro selection, experiments. The details of molecular recognition associated with, targeting by rev of its high affinity RNA binding sites open new, opportunities for structure-based drug design strategies.

A combined NMR-molecular dynamics approach has been applied to determine the solution structure of a HIV-1 17-mer rev peptide bound to its 35-mer high affinity RNA aptamer binding site. Complex formation involves adaptive binding with the alpha-helical arginine-rich basic rev peptide targeting a widened RNA major groove centred about adjacent G.A and reversed A.A mismatches. We have also identified a U AU triple in the aptamer complex with the Hoogsteen-paired uracil base sandwiched between two arginine side chains. The intermolecular contacts identified in the aptamer complex readily account for the consequences of peptide and RNA mutations, as well as the results of previous in vitro selection experiments. The details of molecular recognition associated with targeting by rev of its high affinity RNA binding sites open new opportunities for structure-based drug design strategies.

==About this Structure==

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[[Category: Human immunodeficiency virus 1]]

[[Category: Single protein]]

[[Category: Ellington, A.D.]]

[[Category: Ellington, A D.]]

[[Category: Gorin, A.]]

[[Category: Patel, D.J.]]

[[Category: Patel, D J.]]

[[Category: Ye, X.]]

[[Category: adaptive binding of peptide and rna in complex]]

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[[Category: uau base triple]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-A combined NMR-molecular dynamics approach has been applied to determine, the solution structure of a HIV-1 17-mer rev peptide bound to its 35-mer, high affinity RNA aptamer binding site. Complex formation involves, adaptive binding with the alpha-helical arginine-rich basic rev peptide, targeting a widened RNA major groove centred about adjacent G.A and, reversed A.A mismatches. We have also identified a U AU triple in the, aptamer complex with the Hoogsteen-paired uracil base sandwiched between, two arginine side chains. The intermolecular contacts identified in the, aptamer complex readily account for the consequences of peptide and RNA, mutations, as well as the results of previous in vitro selection, experiments. The details of molecular recognition associated with, targeting by rev of its high affinity RNA binding sites open new, opportunities for structure-based drug design strategies.
+A combined NMR-molecular dynamics approach has been applied to determine the solution structure of a HIV-1 17-mer rev peptide bound to its 35-mer high affinity RNA aptamer binding site. Complex formation involves adaptive binding with the alpha-helical arginine-rich basic rev peptide targeting a widened RNA major groove centred about adjacent G.A and reversed A.A mismatches. We have also identified a U AU triple in the aptamer complex with the Hoogsteen-paired uracil base sandwiched between two arginine side chains. The intermolecular contacts identified in the aptamer complex readily account for the consequences of peptide and RNA mutations, as well as the results of previous in vitro selection experiments. The details of molecular recognition associated with targeting by rev of its high affinity RNA binding sites open new opportunities for structure-based drug design strategies.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Human immunodeficiency virus 1]]
 [[Category: Single protein]]
-[[Category: Ellington, A.D.]]
+[[Category: Ellington, A D.]]
 [[Category: Gorin, A.]]
-[[Category: Patel, D.J.]]
+[[Category: Patel, D J.]]
 [[Category: Ye, X.]]
 [[Category: adaptive binding of peptide and rna in complex]]
@@ Line 24: / Line 24: @@
 [[Category: uau base triple]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:03:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:52 2008''