Sandbox Reserved 702: Difference between revisions
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The second magnesium ion, that is coordinated with the oxygens of the phosphates of ATP, facilitates the bond breakage between α and β-phosphates. Indeed, it stabilizes the resulting negative charges. The stabilisation of the negative charged intermediate is also performed by different positive charged residues: arginine 329, lysine 353, lysine 372 and lysine 346. | The second magnesium ion, that is coordinated with the oxygens of the phosphates of ATP, facilitates the bond breakage between α and β-phosphates. Indeed, it stabilizes the resulting negative charges. The stabilisation of the negative charged intermediate is also performed by different positive charged residues: arginine 329, lysine 353, lysine 372 and lysine 346. | ||
The products, pysophosphate and cyclic AMP dissociate through different solvent accessible channels. It does not require the exposure of the active site to the solvent. However, the magnesium ions cal buid electrostatic interactions with the products and thus impair their dissociation. The binding of a water molecule to the ions is competitive with thier binding to reaction products and breaks the electrostatis links. That is why it is important that the solvant has access to the active site. | |||
