1usi: Difference between revisions

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==Overview==

The periplasmic leucine-binding protein is the primary receptor for the, leucine transport system in Escherichia coli. We report here the structure, of an open ligand-free form solved by molecular replacement and refined at, 1.5-A resolution. In addition, two closed ligand-bound structures of the, same protein are presented, a phenylalanine-bound form at 1.8 A and a, leucine-bound structure at a nominal resolution of 2.4 A. These structures, show the basis of this protein's ligand specificity, as well as, illustrating the conformational changes that are associated with ligand, binding. Comparison with earlier structures provides further information, about solution conformations, as well as the different specificity of the, closely related leucine/isoleucine/valine-binding protein.

The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.

==About this Structure==

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[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Luck, L.A.]]

[[Category: Luck, L A.]]

[[Category: Magnusson, U.]]

[[Category: Mowbray, S.L.]]

[[Category: Mowbray, S L.]]

[[Category: Salopek-Sondi, B.]]

[[Category: PHE]]

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[[Category: x-ray crystallography]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:51 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The periplasmic leucine-binding protein is the primary receptor for the, leucine transport system in Escherichia coli. We report here the structure, of an open ligand-free form solved by molecular replacement and refined at, 1.5-A resolution. In addition, two closed ligand-bound structures of the, same protein are presented, a phenylalanine-bound form at 1.8 A and a, leucine-bound structure at a nominal resolution of 2.4 A. These structures, show the basis of this protein's ligand specificity, as well as, illustrating the conformational changes that are associated with ligand, binding. Comparison with earlier structures provides further information, about solution conformations, as well as the different specificity of the, closely related leucine/isoleucine/valine-binding protein.
+The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Luck, L.A.]]
+[[Category: Luck, L A.]]
 [[Category: Magnusson, U.]]
-[[Category: Mowbray, S.L.]]
+[[Category: Mowbray, S L.]]
 [[Category: Salopek-Sondi, B.]]
 [[Category: PHE]]
@@ Line 24: / Line 24: @@
 [[Category: x-ray crystallography]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:04:24 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:51 2008''